. (3) Justice Fachin issued a provisional ruling
Several studies on the structure of the catalytic domain of the MCR-1 protein have demonstrated that the phosphoethanolamine
transferase is a zinc metalloprotein [94-96], and that zinc deprivation could reduce the colistin MIC in E.
Additional laboratory studies that support the diagnosis include elevated serum pyridoxal 5'-phosphate (vitamin B6) and elevated serum or urine phosphoethanolamine
Raetz, "A phosphoethanolamine
transferase specific for the outer 3-deoxy-D-manno-octulosonic acid residue of Escherichia coli lipopolysaccharide.
(iv) Ethanolamine and phosphoethanolamine
inhibit mitochondrial respiration in a dose-dependent manner by an unknown mechanism .
van Golde, "Immunological characterization, lipid dependence, and subcellular localization of CTP: phosphoethanolamine
cytidylyltransferase purified from rat liver.
THLE-3 cells were cultured in Bronchial Epithelia Cell Growth Medium (Clonetics BEGM bullet kit: CC3170, Lonza, Walkersville, MD, USA) that includes 500 ml basal medium and separate frozen additives from which we discard the gentamycin/amphotericin and Epinephrine and to which we add extra 5 ng/ml EGF, 70 ng/ml Phosphoethanolamine
. HUVECs were cultured in an endothelial basal medium-2 (EBM-2) supplemented with EGM-2 kit (Lonza).
Otherwise, the acute form of glutamate neurotoxicity has been associated to depolarization-induced influx of [Cl.sup.-] (Rothman et al, 1985) and a blockade of swelling-activated chloride channels was shown to be cerebro-protective and to reduce levels of ischemia-induced efflux of aspartate, glutamate, taurine and phosphoethanolamine
from cerebral cortical cells (Phillis et ah, 1998).
Diagnostic findings include defective bone mineralisation, reduced levels of serum alkaline phosphatase, increased levels of serum calcium, excretion of phosphoethanolamine
in the urine and stress fractures.
BEAS-2B cells (passages 70-80) were grown on tissue culture-treated Costar plates in keratinocyte basal medium supplemented with 30 [micro]g/ ml bovine pituitary extract, 5 ng/mL human epidermal growth factor, 500 ng/mL hydrocortisone, 0.1 mM ethanolamine, 0.1 mM phosphoethanolamine
, and 5 ng/mL insulin.
CRP has the highest affinity to phosphocholine residues, but it can also bind to phosphoethanolamine
, chromatin, histones, fibronectin, laminin and polycations (9).
The t365 mutant is impaired in the S-adenosyl-L-methionine phosphoethanolamine
N-methyltransferase (PEAMT) gene.