Insulinotropic effect of cinnamaldehyde on transcriptional regulation of pyruvate kinase, phosphoenol pyruvate
carboxykinase and GLUT4 translocation in experimental diabetic rats.
Liver biopsy samples were collected in screw capped tubes and transferred in liquid nitrogen to the laboratory and stored at -80[degrees]C until measurement of mRNA expression of gluconeogenic enzymes, namely cytosolic phosphoenol pyruvate carboxykinase (PEPCK-C; EC 4.
Regulation of phosphoenol pyruvate carboxykinase (GTP) gene expression.
These transformed cells were subjected to microarray analysis and one of the differentially expressed gene that was identified was enolase-2 (ENO-2) which is a glycolytic enzyme that catalyses the conversion of 2-phosphoglycerate to phosphoenol pyruvate
1986, "Compatible solutes and their effects on phosphoenol pyruvate
carboxylase of C4-halophytes," Plant.
Chemicals: Quercetin dihydrate and phosphoenol pyruvate
, kits for the assay of PTP and PTK were purchased from Sigma-Aldrich Chemicals Pvt Ltd, Bangalore, India.
2), 50 mM KCl, 5 mM MgC12, 10 mM phosphoenol pyruvate
, 2 mM ADP, 0.
Rutgers University (New Brunswick, NJ) has patented materials and methods relating to mildly polar fluid extracts of plant materials, such as Artemisia plant species, useful in methods for treating diabetes and methods for modulating the activity of glucagon-like peptide-1, and in methods for modulating phosphoenol pyruvate
carboxykinase activity in a diabetes-specific manner.
Pyruvate kinase (PK) catalyzes the irreversible phosphoryl group transfer from phosphoenol pyruvate
to ADP, yielding pyruvate and ATP.