coli colonies harboring phagemid
were picked and grown in 2x YT medium containing 100 [micro]g/ml ampicillin, 0.05% glucose, and 1 mM isopropyl-[beta]-thiogalactopyranoside at 30[degrees]C overnight.
The PCR-amplicon was cloned into the KpnI and SacI endonuclease restriction sites of phagemid
pBSSKII- (Invitrogen) and transcripts were generated by run off in vitro transcription from linearized plasmid templates using T7-RNA polymerase.
Louis, MO) and the ZAPII phagemid
(Stratagene, La Jolla, CA), according to the manufacturers' instructions.
Other minor coat proteins, pVI, pVII, or pIX, have been used in phagemid
format, which will have mosaic display of inserted foreign peptides [39,40].
TG1 cells were transformed with pMES4 phagemid
containing the coding sequence of two anti-GFP specific Nbs, Nb07 and 08, which were previously retrieved by phage display from a DNA library prepared from camel immunized with GFP (in press).
- pIII fusion phagemid
for construction of peptide library;
rescuing and the Fab displayed library screening were conducted according to the protocol described .
The pBSK phagemid
vector was linearized by EcoRV (Medox Biotech, India) in order to generate termini GATATC.
The human antibody company, Affitech AS (Oslo, Norway) announced that the European Patent Office has granted an European patent on the use of phagemid
particles having an antibody-coliphage pIII fusion protein, to identify tumor associated antigens on cells by negative selection.
A hemocyte phagemid
unidirectional cDNA library, constructed in the ZAP Expression vector following the manufacturer's recommendations (Stratagene, La Jolla, CA), was previously reported (Jimenez-Vega & Vargas-Albores 2005).
Single-stranded shuttle phagemid
for mutagenesis studies in mammalian cells: 8-oxoguanine in DNA induces targeted G.C[right arrow] T.A transversions in simian kidney cells 50.
Site directed mutagenesis to remove the 5' stop codon was done using the Muta-Gene Phagemid
In Vitro Mutagenesis Version 2 kit (Bio-Rad Laboratories, Hercules, CA).