Xia P Intracytoplasmic sperm injection: correlation of oocyte grade based on polar body, perivitelline
space and cytoplasmic inclusions with fertilization rate and embryo quality.
For some species, particularly those species with a large perivitelline
space, such as Atlantic menhaden (Brevoortia tyrannus), egg shrinkage appears to differ between ethanol and formalin preservation, limiting comparisons of egg-size ranges among our data and historical data.
The onset of oocyte activation are vitellus ink, release discharge and migrate into the perivitelline
Oocytes with intact cell membrane and clear perivitelline
space were selected for future use.
Artificial insemination was performed in 3 females; 2 eggs laid after artificial insemination had spermatozoa present on the perivitelline
layer, suggesting the possible success of the insemination technique.
Variations of microinsemination in clinical use include: zona pellucida drilling, (30) partial zona dissection, (31) and zona opening, (32,33) all of which promote the passage of sperm through the zona pellucida; and subzonal sperm insertion,34) by which motile sperm are injected into the perivitelline
space using a micropipette.
Space (PVS) was small with limited numbers of microvilli.
Morphometric characterization of the eggs was carried out by measuring the diameter of each egg (DE), the perivitelline
space (PS) and yolk diameter (YD).
A universal characteristic of OGPs is their association with the zona pellucida and perivitelline
space of oocytes and embryos, with the possible exception of the mouse zona pellucida.
The donor cell is not injected into the denucleated oocyte, but is placed in the perivitelline
space, whereafter it is combined with the oocyte after electroporation.
Non-fertilized embryos were easily distinguished by the absence of the perivitelline
membrane space between the egg cell membrane and egg shell chorion (Figure 1A).
Maturation rate in vitro of oocytes are assessed by various methods like staining the oocytes (M-II stage), identification of extruded first polar body in the perivitelline
space and degree of expansion of cumulus cell mass (Raghu et al.