coli cells grown on normal medium showed the cell wall composed of outer and cytoplasm membranes enclosing a periplasmic space, the nucleoid containing fine DNA fibril and the cytoplasm containing numerous ribosomes.
coli cells grown on NaCl-betaine containing medium appeared normal, displaying intact and distinct outer membrane and cytoplasmic membrane enclosing a periplasmic space.
Periplasmic proteins are proteins secreted into the periplasmic space
located between the outer and inner membranes of E.
In this system, the signal sequence, pelB, is responsible for the secretion of protein into the periplasmic space
Similarly, Shiga toxin was also found to be released as OMVs from periplasmic space
of Shigella dysenteriae and that the secretion was induced by an antimicrobial compound, mitomycin C (43).
Although disulfide bond formation usually takes place in periplasmic space
, tPA secreted in the periplasm of E.