periodic acid-Schiff stain

(redirected from Periodic acid Schiff)

per·i·od·ic ac·id-·Schiff stain (PAS),

(shif),
a tissue-staining procedure in which 1,2-glycol groupings are first oxidized with periodic acid to aldehydes that then react with the sulfite leucofuchsin reagent of Schiff and turn red-violet; strong staining occurs with polysaccharides, such as glycogen, and mucopolysaccharides of epithelial mucins, basement membranes, and connective tissue.
Synonym(s): PAS stain

Schiff,

Hugo, German chemist in Florence, 1834-1915.
Kasten fluorescent Schiff reagents - see under Kasten
ninhydrin-Schiff stain for proteins
periodic acid-Schiff stain - a tissue-staining procedure. Synonym(s): PAS stain
Schiff base - condensation products of aldehydes and ketones with primary amine. Synonym(s): aldimine
Schiff reagent - used for aldehydes and in histochemistry to detect polysaccharides, DNA, and proteins.

per·i·od·ic ac·id-Schiff stain

(PAS) (pērē-odik asid shif stān)
A clinical tissue-staining procedure.
References in periodicals archive ?
Several studies have suggested that Periodic acid Schiff staining of nail clippings may be a very sensitive method for diagnosis of onychomycosis7,10,11.
Pulmonary alveolar proteinosis (PAP) is a rare lung disease where periodic acid Schiff (PAS)-positive eosinophilic material accumulates in the alveoli of the lungs.
Gomori methenamine silver (GMS) and periodic acid schiff stain (PAS) stains were performed and highlighted thin filamentous organisms.
capsulatum-like yeasts were demonstrated by Periodic Acid Schiff stain.
Slides were stained with Haematoxylin and Eosin, Papanicolaou, Periodic Acid Schiff's and Gomori's methenamine silver stains.
In the cytological examination of BALF, dense acellular eosinophilic amorphous material (Photo 3) and periodic acid Schiff (PAS)-stained macrophages (Photo 4) were seen.
The specimens are rehydrated in 70% ethyl alcohol and then placed successively in periodic acid Schiff reagent, sodium metasulfite, Gill's haematoxylin, and Scott's tap water substitute for 2 minutes each, rinsing in two changes of tap water in between each step.
Staining a smear of aspirated material with mucus specific stain periodic acid Schiff (PAS) confirmed saliva.
Regions Techniques Proximal Medial Distal Intestine Intestine Intestine Periodic Acid Schiff (+) to (+ +) (++) to (+++) (PAS) (+++) Alcian Blue (AB) (+ +) to (+ + +) (++) to (+++) pH 1.0 (+++) PAS/AB pH 1.0 (+) (+) to (+ +) (+) The positive reaction to the dye is represented by "+" and negative reaction by "-" weak reaction to the dye is represented by (+), intermediate reaction by (+ +) and intense reaction by (+++).
Periodic acid Schiff reaction (PAS).--Carbohydrate and glycoprotein deposits in sections of spider abdomens were localized by hydrolysis for 10 min in 0.5% periodic acid in distilled water, followed by a 10 min wash in running tap water and 15 min of staining in freshly prepared 1% Schiff reagent.

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