qPCR results showed a significant decrease in Bifidobacterium, Lactobacillus and Clostridium leptum sub group (*P<0.05) and non-significant decrease and increase in Bacteroides vulgatus and Peptostreptococcus productus, respectively.
Real-time PCR was performed by using primers of Clostridium leptum sub group, Bacteroides vulgatus, Peptostreptococcus productus, Lactobacillus and Bifidobacterium genera for quantitative evaluation of gut microbiota of asthma patients.
To determine the copy number of Clostridium leptum sub group, Peptostreptococcus productus, Bacteroides vulgatus, Lactobacillus and Bifidobacterium genus in each DNA samples, in same experiment, fluorescent signals were calculated, averaged and compared with standard curve that were generated with standard DNA from six serial dilutions in linear range of the program (Ma et al., 2011; Wu et al., 2010).
The Bifidobacterium, Peptostreptococcus productus, Bacteroides vulgatus, Clostridium leptum and Lactobacillus were quantified by real time PCR.
While Peptostreptococcus productus and Bacteroides vulgatus were non-significantly increased, and decreased in diseased group as compare to healthy controls, respectively.
Moreover, there was non-significant increase and decrease of Peptostreptococcus productus and Bacteroides vulgatus respectively, this non-significant decrease of Bacteroides vulgatus in asthma group is also in line with previous studies on diabetes type II patients and metabolic endotoxemia initiates obesity and insulin resistance, which showed non-significant decrease of Bacteroides vulgatus (Wu et al., 2010; Cani et al., 2007).
While, Peptostreptococcus productus, is a reductive acetogenic strain, with its continuous administration, competes with methanogens for H2 in the gut microbial ecosystem due to its reductive acetogenic character (Nollet et al., 1997).
Nowadays, different bacteria such as Butyribacterium methylotrophicum, Eubacterium callanderi, and Peptostreptococcus productus
and the strains Eubacterium limosum, Ruminococcus productus, Clostridium scindens, Peptostreptococcus productus
SECO-Mt75m3, and Eggerthella lenta SECO-Mt75m2 have been involved in the production of enterolignans [65,118].
 obtained the fermentation parameters of Peptostreptococcus productus on gaseous substrates (CO, [H.sub.2]/C[O.sub.2]).
Gaddy, "Fermentation parameters of Peptostreptococcus productus on gaseous substrates (CO, [H.sub.2]/C[O.sub.2])," Archives of Microbiology, vol.