patch clamping

(redirected from Patch clamp)
Also found in: Wikipedia.

patch clamp·ing

a technique used in the study of ion channels in which the movement of ions across a small patch of isolated membrane is measured when the membrane is electrically polarized or hyperpolarized and maintained at that potential.
Synonym(s): patch clamp
Mentioned in ?
References in periodicals archive ?
"Our ultra-small, flexible, nanowire probes could be a very powerful tool as they can measure intracellular signals with amplitudes comparable with those measured with patch clamp techniques; with the advantage of the device being scalable, it causes less discomfort and no fatal damage to the cell (cytosol dilation).
Cell-attached patch clamp recordings were performed on single isolated disc tube feet cells in suspension and visualized on an inverted microscope (Eclipse TE2000, Nikon, Minato, Tokyo) with a x40 objective.
Subsequent to the observations by Katz and colleagues, quantum analysis was applied to mammalian central nervous system (CNS) synapses in the 1990s, when the patch clamp technique was successfully applied to slice preparations.
The patch clamp technology is considered as the gold standard technique in this field.
The company said the Dynaflow Resolve system is considered by many, to be the premier secondary ion channel screening platform on the market, offering high time resolution for any cell type, any ion channel, and in any patch clamp configuration.
Spherically shaped cells were selected for whole-cell patch clamp recording, using an Axopatch 200B amplifier (Axon Instruments, USA) and the output was digitized with a Digidata 1332A converter and pClAMP 9 software (Axon Instruments).
The early studies estimated that pulsed electric fields cause cell membrane permeabilization opening big pores in cell membrane, but currently, patch clamp technology revealed that ultrashort intensive pulsed electrictric field opens small plasma membrane pores [33, 49, 53].
RFP-expressing cells in the slices were identified using a wide-band excitation filter and visualized for whole-cell patch clamp recordings using infrared differential interference contrast video microscopy (BX50WI; Olympus).
Whole-Cell Patch Clamp. Chinese hamster ovary (CHO) cells stably expressing LTCCs were purchased from Ice Ion Channel Explorer (ICE-CHO- [Ca.sub.v]1.2, Beijing, China) and cultured in a standard manner [22].
Their topics include applying immunofluorescence to analyze connexin distribution and trafficking, the patch clamp analysis of gap junction channel properties, recording gap junction-mediated synaptic transmission in vivo at mixed synapses on the goldfish Mauthner cells, assessing connexin hemichannel function during ischemic injury and reperfusion, the functional characterization of connexin hemichannels using Xenopus oocytes and the two-electrode voltage clamp technique, and methods to examine the role of gap junction and pannexin channels in HIV infection.