The PON1 activity was determined with 2 mmol/L of paraoxon
(Sigma Chemical Co.
At an absorbance of 412 nm at 37AoC the percentage of paraoxon
hydrolysis (diethyl-p-nitrophenyl phosphate) was measured.
An example of this phenomenon is paraoxon
, the active metabolite of the pesticide parathion, which is metabolized by enzymes with native lactonase activity (Khersonsky and Tawfik 2005).
Foi utilizado acido ditiobisnitrobenzoico (DTNB), iodeto de acetiltiocolina, iodeto de pralidoxima e paraoxon
do fabricante Sigma- Aldrich[R] e foi utilizado o leitor de microplacas por espectroscopia do fabricante Molecular Devices[R], disponivel no laboratorio do Instituto de Biologia do Exercito.
Adverse outcome pathways during zebrafish embryogenesis: A case study with paraoxon
1988) Role of genetic polymorphism of human plasma paraoxonase/arylesterase in hydrolysis of the insecticide metabolites chlorpyrifos oxon and paraoxon
PON1 activity was measured by spectrophotometric evaluation of 4-nitrophenol, which is the enyzmatic product of the substrate paraoxon
(O, O-diethyl-Op-nitrophenyl phosphate; Sigma Co.
The Q alloenzyme is less efficient in hydrolyzing paraoxon
but more efficient toward oxidized lipoproteins than the R alloenzyme .
Rodriguez H, Espinoza-Navarro O, Silva I, Needham D, Castro M, Sarabia L, Inostroza J, Jimenez L (2011) The effect of paraoxon
on spermatogenesis in Dugesia gonocephala from the Chilean Altiplano; proliferation and apoptosis.
The activities of paraoxonase (PON) and arylesterase (ARE) were estimated spectrophotometrically with the use of paraoxon
and phenyl acetate as respective substrates according to previously described methods [24, 25].
Human serum paraoxonase/aryl esterase (PON 1), (2) is a calcium dependent enzyme that hydrolyses organophosphates such as paraoxon
, diazoxon, sarin, and soman, and also arylesterases such as phenyl acetate.
The PONase activity was measured using paraoxon
(Sigma Chem, USA) as substrate and the increase in absorbance at 412 nm was determined, because of 4 -nitrophenol formation.