Paramphistomum cervi (Trematoda: Digenea: Paramphistomatidae), the representative species of the genus Paramphistomum, has adult flukes that customarily inhabit the rumen and immature worms that parasitize the gallbladder and reticulum of ruminants, including cattle, sheep, goat, and some wild mammals [1, 2].
To identify novel genetic markers for studying intraand interspecific variation in the Paramphistomatidae and to further study the systematics and phylogenetics of Digenea trematodes, the present study determined and characterized the complete rDNA sequence of P cervi, studied the intraspecific variation, and reconstructed the phylogenetic relationship of P.
The intraspecies sequence variation in each of these rDNA regions among the five individual adults and the interspecies sequence differences of the ITS2 rDNA within the family Paramphistomatidae were determined using the MegAlign procedure in DNASTAR 5.0 .
However, a comparative analysis revealed that the interspecific differences in ITS2 among members of the family Paramphistomatidae were 1.4-6.3%.
The present study reported the complete 5.8S and 28S rDNA sequences, which are the only representative sequences of the family Paramphistomatidae, of P.
Because no other IGS rDNA sequences of the family Paramphistomatidae were available in GenBank, the interspecific differences were not examined.
2% 1% 1% 5% Macroderoididae Glypthelmins quieta 0% 0% 26% 0% Macroderoides typicus 37% 40% 12% 62% Paramaroderoides echinus 23% 47% 13% 36% Ochetosomatidae 18% 37% 9% 72% Dasymetra conferta Ochetosoma aniarum Ochetosoma ellipticum Pneumatophilus variabilis Paramphistomatidae
8% 3% 5% 2% Allassostomoides chelydrae Megalodiscus intermedius Telorchidae 15% 8% 10% 80% Auridistomum chelydrae Protenes angustus Telorchis clavi Telorchis corti Nematode Larvae: Dioctophymidae Eustrongylides sp.