Papanicolaou stain

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Pa·pa·ni·co·la·ou stain

a multichromatic stain used principally on exfoliated cytologic specimens and based on aqueous hematoxylin with multiple counterstaining dyes in 95% ethyl alcohol, giving great transparency and delicacy of detail; important in cancer screening, especially of gynecologic smears.


George N., Greek-U.S. physician, anatomist, and cytologist, 1883-1962.
Papanicolaou examination
Papanicolaou smear - Synonym(s): Pap smear
Papanicolaou smear test - Synonym(s): Pap test
Papanicolaou stain - a multichromatic stain used in cancer screening, especially of gynecologic smears.
Papanicolaou test - Synonym(s): Pap test
Pap smear - vaginal or cervical cells obtained for cytological study. Synonym(s): Papanicolaou smear
Pap test - examination of cells stained with Papanicolaou stain. Synonym(s): Papanicolaou smear test; Papanicolaou test
References in periodicals archive ?
4 Automatic system for staining and mounting of slides diagnostic accordance with the Papanicolaou stain.
The main components of Papanicolaou stain includes basic dye-hematoxylin, which stains the nucleus; and three acid dyes-light green, eosin, and Orange G, which stain the cytoplasm.
Smears were stained with Romanosky and papanicolaou stain.
coli were also seen in the Papanicolaou stain (Fig.
The following sperm, stained with the Papanicolaou stain would be classified as normal:
A low cost Papanicolaou stain protocol for cancer screening [Abstract].
The specimen is smeared on a microscope slide, followed by specific staining of the deposited cells on the slide with the Papanicolaou stain.
Another set of slides was fixed in 950 mL/L alcohol and stained later with Papanicolaou stain.
4) The spherical, thick-walled, broad-based yeast cells (diameter: 10 to 20 [micro]m) can be identified in frozen sections or by any of the fungal-wall stains (potassium hydroxide, Gomori, and PAS) and on Papanicolaou stain.
FNA of the hepatic mass was performed with a 22G spinal needle and smears were prepared on site and fixed in 95% ethanol and stained with Papanicolaou stain.
B, In this field, only the polymorphous lymphoid population is present (ThinPrep Pap test; Papanicolaou stain, original magnifications 3400 [A] and 3200 [B]).
Although prestained slides (Testsimplets, Irvine Scientific, Santa Ana, CA), quick stains, including the Diff-Quik (Fisher, Pittsburgh, PA), and modified Pap (Sage BioPharma, Bedminster, NJ) are acceptable for assessing sperm morphology, the Papanicolaou stain is recommended for clarity of reading.