Subsequent to initial changed membrane permeability or final cell disruption that accompanies necrotic death, cytosolic enzymes such as ALT and AST are released into the blood serum ALT and AST levels were measured after PZA
Currently, there are two FDA-cleared platforms for PZA
susceptibility testing; the BACTEC[TM] MGIT[TM] (Becton Dickinson ("BD"), Sparks, MD) system (henceforth designated as "MGIT system") and the Versa-TREK MYCO TB[TM] (TREK Diagnostic Systems, Cleveland, OH) system.
Although the CLSI guidelines 2003 have recommended the use of BACTEC Mycobacterial Growth Indicator Tube (MGIT) 960 for PZA
DST, the technique does not seem to yield reliable/ reproducible results (9, 10, 11).
Basification of PZA
4 to Zwitterionic Anionic Polyelectrolyte (ZAPE) 7.
In patients with a baseline ALT > 120 IU/l or total bilirubin > 40 [micro]mol/l, it is recommended to start standard TB treatment and monitor closely, or to omit PZA
initially in severe cases.
Previously, the Johns Hopkins group identified mutations in the pncA gene and the rpsA gene as the primary causes for PZA
We undertook this study to compare MGIT 960 PZA
susceptibility test with pyrazinamidase activity assays in 136 clinical isolates of M.
In the June 6 Nature Medicine, Ying Zhang and Angelo Scorpio of Johns Hopkins University School of Hygiene and Public Health in Baltimore report that they have identified a TB gene, pncA, that codes for an enzyme that converts the TB-fighting PZA
into an acid lethal to the bacterium.
Washington, Aug 12 (ANI): Scientists have conducted a study on how critical drug Pyrazinamide (PZA
) works with other medications to kill TB bacteria.
Most importantly, treatment regimens should not depend on the results of drug susceptibility testing (DST) for ethambutol (EMB) or pyrazinamide (PZA
One of the recommended treatments for latent tuberculosis infection (LTBI) is a 9-month regimen of isoniazid (INH); a 2-month regimen of rifampin (RIF) and pyrazinamide (PZA
) is an alternative in some instances.