PUM1

PUM1

A gene on chromosome 1p35.2 that encodes a sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. PUM1 may support stem cell proliferation and self-renewal by regulating translation of key transcripts. It is expressed in the brain, heart, kidneys, muscle, intestine and stomach and downregulated in keratinocytes in response to UVB irradiation.
References in periodicals archive ?
The amount of target gene was obtained by normalizing to the endogenous reference Pumilio RNA-Binding Family Member 1 (PUM1) and was expressed relatively to vehicle-treated cells set at 1.
Over increasing durations of hyperglycemia exposure, SP1 mRNA levels were evaluated in HRECs (Figure 4(a)) and ARPE-19 cells (Figure 4(b)) after normalization relative to the mRNA levels of Pumilio homolog 1 (PUM1) and peptidylprolyl isomerase A (PPIA), respectively.
SP1 mRNA levels were normalized relative to the mRNA levels of PUM1 and PPIA in HRECs and RPE cells, respectively.
haESCs have also been applied to screen for genes required for the exit from self-renewal, and novel differentiation factors including Zfp706 and Pum1 were discovered [45].
miR-221 and In arrested cells, Pum1 is unable to [154] miR-222 bind to 3'UTR of target mRNA and cannot open its loop structure and therefore restricts miRNA binding.
PUM1 was previously found to be the most stable endogenous control mRNA and was therefore used as a reference gene to normalize expression levels of target mRNAs [22].
Identification and gene expression profiling of the Pum1 and Pum2 members of the Pumilio family in the chicken.
To correct for differences in sample quality and cDNA input, we adjusted copy numbers to the arithmetic mean of 5 housekeeper genes [[ACTB.sup.8] ([beta]-actin), PSMC4 (proteasome 26S subunit, ATPase, 4), PUM1 (pumilio homolog 1, Drosophila), MRPL19 (mitochondrial ribosomal protein L19), and SF3A1 (splicing factor 3a, subunit 1, 120 kDa)].