Cosin-Roger et al., "Prdx6
deficiency ameliorates DSS colitis: relevance of compensatory antioxidant mechanisms," Journal of Crohn's & Colitis, vol.
Upregulation by Curcumin Attenuates Ischemic Oxidative Damage via SP1 in Rats after Stroke.
Our study reported similar findings, which demonstrated that using a panel of six TAAs (i.e., BMI-1, PRDX6
, NY-ESO-1, p53, HSP70, and MMP-7) could distinguish patients with early ESCC from normal controls, with a sensitivity/specificity of 45%/95% .
Moreover, in P1 cells, resveratrol treatment induced an upregulation of PRX1 and a downregulation of PRDX6
, which uses glutathione as the physiological reductant, saving the amount of the glutathione for other activities.
The key targets in dioscin-induced apoptosis were PRDX1 and PRDX6
(Wang et al., 2012).
Plasma levels of peroxiredoxin isoforms in non-diabeitc healthy control subjects and diabetic patients Non-diabetic Diabetic subjects (n = 25) patients (n = 53) PRDX1 (ng/mL) 16.77 [+ or -] 3.899 21.92 [+ or -] 5.77 * PRDX2 (ng/mL) 20.37 [+ or -] 8.61 36.61 [+ or -] 14.966 * PRDX4 (pg/mL) 2696 [+ or -] 1972 3835 [+ or -] 1454 * PRDX6
(pg/mL) 238 [+ or -] 111 311 [+ or -] 111 * Data are reported as means [+ or -] SD.
Various novel proteins have also been shown to be direct substrates of p38[alpha], including Ahnak, Iws1, Grp78, Pgrmc, Prdx6
, and Ranbp2 .
Peroxiredoxin 6 (Prdx6
) is the sixth mammalian peroxiredoxin isoform and has a single conserved cysteine residue .
Primers for rat genes used to verify the array-based gene expression, by rt-PCR Gene name Forward primer 5'-3' Reverse primer 5'-3' Hmger CCATGGCTGGTAGCATAGG GTACAGCTGATGTATAAGTCTTC Cyp17a ACGGTGGGAGACATCTTTGG TGGTCAATCTCCTTTTGGATCTTC Ndufv2 TGACAGCATCCTGGAGAC GAACTTTCCCAGCTCTGAG Dgat1 GAATGCTGAGTCTGTCACC AGCCATCATGGCTGTGAATG Prdx6
GATTCTCAGAGTGGTTGACTC AGCTCTTTGGTGAAGACTCC LSS CAATCCTGACTACCAGAAG CATTGTAGAGTCGCTCTTG FDFT1 TACCTGTCAAGGCTCCGGAAC GCATCCATCATGAGGGTAACTG PEMT CAGCATCATCCTGCTTCTGAAC CCAAAGTAGTCACCTAGAAAGG 2[beta] microglobulin CAACTTCAACTGCTACG CAGAGCTCCATAGAGCTTGA RNA preparation, reverse transcription and real time PCR
Primers for human GAPDH, SOD1, SOD2, CAT, GPX1, CCS, PRDX6
, FOXO3, CDKN2A, PAK2, TP53, MAPK14, and JUN were designed by using GenBank database sequences and Primer 3 software  (http://bioinfo.ut.ee/primer3-0.4.0/).
A previous report found that the antioxidant protein Prdx6
was increased in the liver of Sig-1R KO animals compared to that of the WT mice .
Some detected no antioxidant proteins [47, 48] while others reported them to contain GPX, GST, PRDX1-3, and SOD2 ; GPX, PRDX5, TRX, and SOD2 ; GST, SOD2, and PRDX6
; and GPX3, PRX1, PRX2, and PRX4 .