Single colony was picked from 58 samples separately and after re-culturing on PPLO agar, all of these samples showed plate growth.
of samples showing turbidity on PPLO broth and plate growth on PPLO agar.
of samples showing turbidity in PPLO broth; C+, No.
The results of different field samples (after primary culture showing whorl formation in PPLO broth) subjected to PCR have been shown in Table V.
All samples were streaked into PPLO media and incubated in anaerobic incubator (New Brunswick, Galaxy 48-S UK) with 5% CO2 at 37AdegC for 3-7 days for the isolation of mycoplasma species as described previously (Shahzad et al., 2012; OIE, 2014).
In the present study Mycoplasma was successfully grown on modified PPLO media for isolation from samples originated from different sources of suspected animals including nasal discharges, pleural fluid and lung tissues.
pneumoniae growing on PPLO
agar (magnification x160)
Ltd., Guangdong, China) containing PPLO
broth, horse serum, and 25% yeast extract, which was added along with penicillin G (1,000 U/ml), thallium acetate (0.025%), glucose (0.5%), and phenol red (0.002%) at pH 7.6.