The bisulfate-treated genomic DNA was then used as a template for the amplification of promoters of GR PPARa
We found that P-AmPk/AMPK levels exhibited similar change pattern as PPAR[alpha] did (Supplementary Figure 1), suggesting that exercise prevented ketogenic diets induced lipid disorders by decreasing lipid storage via PPAR[gamma], while increasing lipid [beta]-oxidation via PPARa
Wan et al., "Effect of miR-34a in regulating steatosis by targeting PPARa
expression in nonalcoholic fatty liver disease," Scientific Reports, vol.
Gene Primers Srebf2 F: 5'-CGCTCCACAGACCAGGATCA-3' R: 5'-TGTCACGAGGCTTTGCACTTG-3' Srebf1 F: 5'-GGAGCCATGGATTGCACATT-3' R: 5'-AGGAAGGCTTCCAGAGAGGA-3' Ppara
F: 5'-GACAAGGCCTCAGGATACCACTATG-3' R: 5'-TTGCAGCTTCGATCACACTTGTC-3' Prkaa1 F: 5'-GGCTCGCCCAATTATGCTG-3' R: 5'-AGAGTTGGCACGTGGTCATCA-3' Acaca F: 5'-CAATCCTCGGCACATGGAGA-3' R: 5'-GCTCAGCCAAGCGGATGTAGA-3' [beta]-actin F: 5'-GGAGATTACTGCCCTGGCTCCTA-3' R: 5'-GACTCATCGTACTCCTGCTTGCTG-3' Srebf1: sterol regulatory element binding transcription factor 1; Srebf 2: sterol regulatory element binding transcription factor 2; Ppara
: peroxisome proliferator-activated receptor alpha; Acaca: acetyl-coenzyme A carboxylase alpha; Prkaa1: protein kinase, AMP-activated, alpha 1 catalytic subunit.
Svalstedt, "Thiazolidinediones (PPAR[gamma] agonists) but not PPARa
agonists increase IRS-2 gene expression in 3T3-L1 and human adipocytes," The FASEB Journal, vol.
Experimental studies and animal models have shown that PFAS can act as agonists of the peroxisome proliferator-activated receptor-alpha (PPARa
), which might lead to liver damage (Intrasuksri et al.
as a therapeutic target in diabetic nephropathy and other renal diseases.
Zeldin et al., "The epoxygenases CYP2J2 activates the nuclear receptor PPARa
in vitro and in vivo," PLoS ONE, vol.
Lee et al., "Liver PPARa
and UCP2 are involved in the regulation of obesity and lipid metabolism by swim training in genetically obese db/db mice," Biochemical and Biophysical Research Communications, vol.
The ability of both CA and FA to prevent HFD-induced hypertriglyceridemia is remarkable and likely to involve i) a direct liver effect to coordinate reduction in the FAS and DGAT-2 expressions during HFD as well as to coordinate the induction of ATGL and PPARa
expression and/or ii) an indirect effect mediated via prevention of obesity.
2006), in addition to antiobesity properties, related to the ability to activate PPARa
in vitro (Bai et al.