We next examined whether TNF-[alpha] or IFN-[gamma] was responsible for the decreased mRNA expression of POT1 identified in SAA patients.
TRF2 is able to bind to and suppress ATM, whereas POT1 prevents activation of ATR .
POT1 has a critical role in all these activities .
In the present study, we detected very low POT1 mRNA expression in patients with SAA.
This involves activation of ATM, which is normally suppressed by TRF2 and/or activation of ATR which is normally suppressed by POT1 .
TNF-[alpha] and IFN-[gamma] are found at high concentrations in SAA and may be responsible for triggering senescence or apoptosis through POT1 and activation of ATM/ATR.
de Lange, "Protection of telomeres through independent control of ATM and ATR by TRF2 and POT1," Nature, vol.
Price, "Pot1 and telomere maintenance," FEBS Letters, vol.
de Lange, "Functional dissection of human and mouse POT1 proteins," Molecular and Cellular Biology, vol.
Counter, "POT1 association with TRF2 regulates telomere length," Molecular and Cellular Biology, vol.
Column GP Relay CAN H CAN L Ground Vbatt J1-1 7b(PS+Sense), 6b (PS+) Power Ground J1-2 * Brake J1-3 9 Accelerator J1-4 10 CAN H J1-5 * CAN L J1-6 * Pot1
Vref J2-1 6 Pot1
Wiper J2-2 5 Pot1
Ground J2-3 7, 8 Pot2 Vref J3-1 2 Pot2 Wiper J3-2 1 Pot2 Ground J3-3 3, 4 Motor + J3-4 12 3b (Load 1) Motor - J3-5 11 2b (Load 2) Other Connections PS + Sense 7a PS + 6a PS - Sense 5a PS - 4a Motor Load + 3a Motor Load - 2a Chassis Ground 1a Switched Chassis Ground 1b * DUT Common * Star Ground 13, 14 5b (PS-Sense), * 4b (PS-) Figure 4.