Phosphorus, Blood

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Phosphorus, Blood

Synonym/acronym: Inorganic phosphorus, phosphate, PO4.

Common use

To assist in evaluating multiple body system functions by monitoring phosphorus levels in relation to other electrolytes. Used specifically to evaluate renal function in at-risk patients.


Serum (1 mL) collected in a gold-, red-, or red/gray-top tube. Plasma (1 mL) collected in green-top (heparin) tube is also acceptable.

Normal findings

(Method: Spectrophotometry)
AgeConventional UnitsSI Units (Conventional Units × 0.323)
0–5 day4.6–8 mg/dL1.5–2.6 mmol/L
1–3 yr3.9–6.5 mg/dL1.3–2.1 mmol/L
4–6 yr4–5.4 mg/dL1.3–1.7 mmol/L
7–11 yr3.7–5.6 mg/dL1.2–1.8 mmol/L
12–13 yr3.3–5.4 mg/dL1.1–1.7 mmol/L
14–15 yr2.9–5.4 mg/dL0.9–1.7 mmol/L
16–19 yr2.8–4.6 mg/dL0.9–1.5 mmol/L
Adult2.5–4.5 mg/dL0.8–1.4 mmol/L
Values may be slightly decreased in older adults due to dietary insufficiency or the effects of medications and the presence of multiple chronic or acute diseases with or without muted symptoms.


Phosphorus, in the form of phosphate, is distributed throughout the body. Approximately 85% of the body’s phosphorus is stored in bones; the remainder is found in cells and body fluids. It is the major intracellular anion and plays a crucial role in cellular metabolism, maintenance of cellular membranes, and formation of bones and teeth. Phosphorus also indirectly affects the release of oxygen from hemoglobin by affecting the formation of 2,3-bisphosphoglycerate. The reabsorption and excretion of phosphorus is largely regulated by the parathyroid glands and the kidneys. Levels of phosphorus are also affected by dietary intake and are dependent on the presence of activated vitamin D for absorption by the intestines. Calcium and phosphorus are interrelated with respect to absorption and metabolic function. They have an inverse relationship with respect to concentration; serum phosphorus is increased when serum calcium is decreased.

Hyperphosphatemia can result in an infant fed only cow’s milk during the first few weeks of life because of the combination of a high phosphorus content in cow’s milk and the inability of infants’ kidneys to clear the excess phosphorus.

This procedure is contraindicated for



  • Assist in establishing a diagnosis of hyperparathyroidism
  • Assist in the evaluation of renal failure

Potential diagnosis

Increased in

  • Acromegaly (related to increased renal absorption)
  • Bone metastases (related to release from bone stores)
  • Diabetic ketoacidosis (acid-base imbalance causes intracellular phosphorus to move into the extracellular fluid)
  • Excessive levels of vitamin D (vitamin D promotes intestinal absorption of phosphorus; excessive levels promote phosphorus release from bone stores)
  • Hyperthermia (tissue damage causes intracellular phosphorus to be released into circulation)
  • Hypocalcemia (calcium and phosphorus have an inverse relationship)
  • Hypoparathyroidism (related to increased renal absorption)
  • Lactic acidosis (acid-base imbalance causes intracellular phosphorus to move into the extracellular fluid)
  • Milk alkali syndrome (increased dietary intake)
  • Pseudohypoparathyroidism (related to increased renal absorption)
  • Pulmonary embolism (related to respiratory acid-base imbalance and compensatory mechanisms)
  • Renal failure (related to decreased renal excretion)
  • Respiratory acidosis (acid-base imbalance causes intracellular phosphorus to move into the extracellular fluid)

Decreased in

    Acute gout (related to decreased circulating calcium in calcium crystal–induced gout; calcium and phosphorus have an inverse relationship) Alcohol withdrawal (related to malnutrition) Gram-negative bacterial septicemia Growth hormone deficiency Hyperalimentation therapy Hypercalcemia (calcium and phosphorus have an inverse relationship) Hyperinsulinism (insulin increases intracellular movement of phosphorus) Hyperparathyroidism (parathyroid hormone [PTH] increases renal excretion) Hypokalemia Impaired renal absorption (decreases return of phosphorus to general circulation) Malabsorption syndromes (related to insufficient intestinal absorption of phosphorus) Malnutrition (related to deficient intake) Osteomalacia (evidenced by hypophosphatemia) PTH-producing tumors (PTH increases renal excretion) Primary hyperparathyroidism (PTH increases renal excretion) Renal tubular acidosis Renal tubular defects (related to decreased renal absorption) Respiratory alkalosis Respiratory infections Rickets (related to vitamin D deficiency) Salicylate poisoning Severe burns Severe vomiting and diarrhea (related to excessive loss) Vitamin D deficiency (related to vitamin D deficiency, which reduces intestinal and renal tubular absorption of phosphorus)

Critical findings

  • Adults
  • Less than 1.0 mg/dL (SI: Less than 0.3 mmol/L)
  • Greater than 8.9 mg/dL (SI: Greater than 2.9 mmol/L)
  • Children
  • Less than 1.3 mg/dL (SI: Less than 0.4 mmol/L)
  • Greater than 8.9 mg/dL (SI: Greater than 2.9 mmol/L)
  • Note and immediately report to the health-care provider (HCP) any critically increased or decreased values and related symptoms.

  • It is essential that a critical finding be communicated immediately to the requesting health-care provider (HCP). A listing of these findings varies among facilities.

  • Timely notification of a critical finding for lab or diagnostic studies is a role expectation of the professional nurse. Notification processes will vary among facilities. Upon receipt of the critical value the information should be read back to the caller to verify accuracy. Most policies require immediate notification of the primary HCP, Hospitalist, or on-call HCP. Reported information includes the patient’s name, unique identifiers, critical value, name of the person giving the report, and name of the person receiving the report. Documentation of notification should be made in the medical record with the name of the HCP notified, time and date of notification, and any orders received. Any delay in a timely report of a critical finding may require completion of a notification form with review by Risk Management.

  • Interventions including IV replacement therapy with sodium or potassium phosphate may be necessary. Close monitoring of both phosphorus and calcium is important during replacement therapy.

Interfering factors

  • Drugs that may increase phosphorus levels include anabolic steroids, β-adrenergic blockers, ergocalciferol, furosemide, hydrochlorothiazide, methicillin (occurs with nephrotoxicity), oral contraceptives, parathyroid extract, phosphates, sodium etidronate, tetracycline (occurs with nephrotoxicity), and vitamin D.
  • Drugs that may decrease phosphorus levels include acetazolamide, albuterol, aluminum salts, amino acids (via IV hyperalimentation), anesthetic agents, anticonvulsants, calcitonin, epinephrine, fibrin hydrolysate, fructose, glucocorticoids, glucose, insulin, mannitol, oral contraceptives, pamidronate, phenothiazine, phytate, and plicamycin.
  • Serum phosphorus levels are subject to diurnal variation: They are highest in late morning and lowest in the evening; therefore, serial samples should be collected at the same time of day for consistency in interpretation.
  • Hemolysis will falsely increase phosphorus values.
  • Specimens should never be collected above an IV line because of the potential for dilution when the specimen and the IV solution combine in the collection container, thereby falsely decreasing the result. There is also the potential of contaminating the sample with the substance of interest if it is present in the IV solution, thereby falsely increasing the result.

Nursing Implications and Procedure


  • Positively identify the patient using at least two unique identifiers before providing care, treatment, or services.
  • Patient Teaching: Inform the patient this test can assist in a general evaluation of body systems.
  • Obtain a history of the patient’s complaints, including a list of known allergens, especially allergies or sensitivities to latex.
  • Obtain a history of the patient’s endocrine, gastrointestinal, genitourinary, and musculoskeletal systems; symptoms; and results of previously performed laboratory tests and diagnostic and surgical procedures.
  • Obtain a list of the patient’s current medications, including herbs, nutritional supplements, and nutraceuticals (see Effects of Natural Products on Laboratory Values online at DavisPlus).
  • Review the procedure with the patient. Inform the patient that specimen collection takes approximately 5 to 10 min. Address concerns about pain and explain that there may be some discomfort during the venipuncture.
  • Sensitivity to social and cultural issues, as well as concern for modesty, is important in providing psychological support before, during, and after the procedure.
  • Note that there are no food, fluid, or medication restrictions unless by medical direction.


  • Potential complications: N/A
  • Avoid the use of equipment containing latex if the patient has a history of allergic reaction to latex.
  • Instruct the patient to cooperate fully and to follow directions. Direct the patient to breathe normally and to avoid unnecessary movement.
  • Observe standard precautions, and follow the general guidelines in Patient Preparation and Specimen Collection. Positively identify the patient, and label the appropriate specimen container with the corresponding patient demographics, initials of the person collecting the specimen, date, and time of collection. Perform a venipuncture.
  • Remove the needle and apply direct pressure with dry gauze to stop bleeding. Observe/assess venipuncture site for bleeding or hematoma formation and secure gauze with adhesive bandage.
  • Promptly transport the specimen to the laboratory for processing and analysis.


  • Inform the patient that a report of the results will be made available to the requesting HCP, who will discuss the results with the patient.
  • Nutritional Considerations: Severe hypophosphatemia is common in elderly patients or patients who have been hospitalized for long periods of time. Good dietary sources of phosphorus include meat, dairy products, nuts, and legumes. To decrease phosphorus levels to normal in the patient with hyperphosphatemia, dietary restriction may be recommended. Other interventions may include the administration of phosphate binders or calcitriol (the activated form of vitamin D).
  • Nutritional Considerations: Vitamin D is necessary for the body to absorb phosphorus. Educate the patient with vitamin D deficiency, as appropriate, that the main dietary sources of vitamin D are cod liver oil and fortified dairy foods such as milk, cheese, and orange juice. Explain to the patient that vitamin D is also synthesized by the body, in the skin, and is activated by sunlight.
  • Reinforce information given by the patient’s HCP regarding further testing, treatment, or referral to another HCP. Answer any questions or address any concerns voiced by the patient or family. Educate the patient regarding access to nutritional counseling services. Provide contact information, if desired, for the Institute of Medicine of the National Academies (
  • Depending on the results of this procedure, additional testing may be performed to evaluate or monitor progression of the disease process and determine the need for a change in therapy. Evaluate test results in relation to the patient’s symptoms and other tests performed.

Related Monographs

  • Related tests include biopsy bone, blood gases, BUN, calcitonin, calcium, calculus kidney stone panel, carbon dioxide, chloride, collagen cross-linked N-telopeptides, CBC WBC count and differential, creatinine, fecal analysis, fecal fat, FDP, glucagon, glucose, GH, insulin, lactic acid, lung perfusion scan, lung ventilation scan, osmolality, osteocalcin, PTH, parathyroid scan, phosphorus urine, potassium, US abdomen, and vitamin D.
  • Refer to the Endocrine, Gastrointestinal, Genitourinary, and Musculoskeletal systems tables at the end of the book for related tests by body system.
References in periodicals archive ?
Finally, the parameters of the regression analysis on PO4 (fairly good primary outcome, no secondary outcome) do not produce additional indications.
Seasonal variation of reactive phosphorus concentrations [micro]M (PO4) at: a) San Juan de la Costa, b) Rancheros del Mar and c) El Mogote during June 2006 to May 2007.
For the M4 and M8 samples a percentage equal to 50% of the CaO and Ca3(PO4)2 components is taken into account.
Temperature, electric conductivity, pH were measured on the spot using respective electrode (CONSORT C931 Turnhout, Belgium) electrochemical analyzer,, while anions such as Nitrate (No3 ), Nitrite (No2 ), Sulfate (SO4 ), Phosphate (PO4) and Chloride (Cl) using (DR2800 spectrophotometer, HACH Company, USA).
Raman spectrum analysis of hydroxyapatite presented a strong peak at 961 [cm.sup.-1] is attributed to [v.sup.1] PO4 symmetric stretching, peak at 600[cm.sup.-1] is of [v.sup.4] PO4 bend (asymmetric), peak at 412 [cm.sup.-1] attributed to [v.sup.2] PO4 asymmetric stretching [25].
For instance, despite of PO4 is of secondary influence like other process in domain "Plan and Organize", as PO3, in the mapping of IT processes to IT governance focus areas, COBIT 4.1 declares PO4 with a low importance and the weighting scheme assigns to it a null weight.
In this study various composition of industrial and municipal wastewater were prepared for analysis of various parameters (BOD COD color removal TSS TDS TKN TP NO3 PO4 and water evaporation rate).
She had normal renal function test, LDH, ALT, AST, Na, Po4, and Mg.
This titration method is very interesting and suited for the flow-injection determination of many other elements such as Cl- and PO4 3-ions.
In 1900 the Swiss adopted the Luger in the .30 Luger chambering, while the German Navy adopted it in 1904 as the PO4 while the Army picked it up in 1908 as the P08--albeit both of the latter were in 9mm Luger instead of .30.