Many homozygous and heterozygous mice, including Cdh23 , Pmca2
, Sodl , Gpxl , Trpv4 , and Hsfl [63, 64] knockout mice, have been shown to be more sensitive to noise-induced hearing loss than the other wild-type strains.
The plasma membrane [Ca.sup.2+]-ATPase2 (PMCA2) is reported to be involved in extrusion of calcium and cerebellar synapse function .
PMCA2 belongs to the family of P-type primary ion transport ATPases characterized by the formation of aspartyl phosphate intermediate during an ATP hydrolysis reaction cycle.
The aim of this study was to investigate whether PMCA2 activity may be involved in Purkinje cell dendritic growth and whether it would be modulating the effects of mGluR1 activation on the development of the Purkinje cell dendritic tree.
To validate the expression of PMCA2 in Purkinje cell dendrites and dendritic spines, rabbit anti-calcium pump PMCA2 ATPase antibody (Abcam, United Kingdom, 1 : 500) was used as a primary antibody and goat anti-rabbit Alexa 488 (Molecular Probes, Invitrogen, 1: 500) was used as a secondary antibody.
The Plasma Membrane [Ca.sup.2+] -ATPase (PMCA2) Is Strongly Expressed in Purkinje Cell Dendrites in Cerebellar Slice Cultures.
In order to verify the expression of PMCA2 independently in Purkinje cell dendrites and dendritic spines in cerebellar slice cultures, we performed immunohistochemistry with anti-PMCA2 antibody on cerebellar slices after 10 DIV.
Chronic Inhibition of PMCA2 by Carboxyeosin Induced a Moderate Reduction of Purkinje Cell Dendritic Tree Size.
Chronic Inhibition of PMCA2 Protects Purkinje Cells from Dendritic Reduction Induced by mGluR1 Stimulation.
The major finding of this study is that PMCA2 inhibition with carboxyeosin by itself reduced the size of the Purkinje cell dendritic tree, but at the same time it had a strong rescuing effect for the dendritic tree from chronic mGluR1 stimulation through DHPG.
Inhibiting PMCA2 by carboxyeosin will inhibit a major [Ca.sup.2+]-extrusion mechanism and would thus result in increased Ca + levels.