cDNA of phospholipase C gamma 2 ( PLCG2 ) were screened by means of PCR amplification of overlapping segments and then Sanger sequencing.
No mutations were detected in exon 3 of NLRP12 or exons 1-33 of PLCG2 .
FACU/ PLCG2 associated antibody deficiency and immune dysregulation (PLAID) is currently thought to be caused by deletions in PLCG2 . PLAID is characterized by CU, autoimmunity, recurrent bacterial infection, and skin granuloma formation.
The NLR and PLCG2 genes may be analyzed in mutation detection studies involving patients with FCU.
(44.) Sims, R., et al, (2017), "Rare coding variants in PLCG2
, ABI3, and TREM2 implicate microglial-mediated innate immunity in Alzheimer's disease," Nature Genetics, 49, 1373-1384.
Between-placenta variability was higher than within-placenta variability for all genes, except for SYN1, AKT2, and PLCG2 (see Supplemental Material, Table S2).
Similarly, the response variable consisted of eight gene expression values per placenta within the SOS cascade (BDNF, SOS1, SOS2, and SYN1) and six gene expression values per placenta within the PLCG cascade (BDNF, PLCG1, and PLCG2).
We found for the PLCG cascade (BDNF, PLCG1, and PLCG2) that [PM.sub.2.5] exposure during the first month (p = 0.001) and the first trimester (p = 0.009) of pregnancy was associated with significantly lower levels of placental gene expression at birth (Table 3).