Methods: From June 2016 to June 2017, six human skins were fixed, paraffin-embedded, and cut into 5 [micro]m-thick sections, followed by costaining for nerve fiber markers protein gene product 9.5 (PGP 9.5), tyrosine hydroxylase (TH) and vasoactive intestinal peptide (VIP), and eccrine sweat gland markers K7, S100P, and K14 by combining standard immunofluorescence with tyramide signal amplification (IF-TSA).
To investigate the differential innervation of secretory coils and ducts, full-thickness skins were costained for nerve fiber markers protein gene product 9.5 (PGP 9.5), tyrosine hydroxylase (TH) and vasoactive intestinal peptide (VIP), and eccrine sweat gland markers by combining standard IF with tyramide signal amplification (IF-TSA).
 structions of thick (30-80 [micro]m) tissue sections IH: PGP 9.5
, CGRP, fast blue Tesarz Rat (n = 8) IH: PGP 9.5
, Rich innervation et al.
Antigen retrieval was performed on sections through immunostaining for PGP 9.5
and c-kit by Tween 20 (0.2% in PBS).
Common for all these methods is the use of immunohistochemistry with a protein gene product 9.5 (PGP 9.5
Essentially, pulp sections were labelled with a mixture of a polyclonal antibody to human TRPV1 raised in rabbit (1:2000; GlaxoSmithKline, Harlow, UK) and either: i) a monoclonal antibody to the general neuronal marker, human protein gene product 9.5 (PGP 9.5
) raised in mouse (1:1000; Ultraclone, Isle of Wight, UK) or ii) a mixture of biotinylated Ulex europaeus agglutinin 1 lectin (UEIL) (1:100; Vector Laboratories, Peterborough, UK), and an antibody raised in mouse against human alpha smooth muscle actin (.SMA) (1:50; Novocastra Ltd, Newcastle Upon Tyne, UK).
The distribution of Kit-ir ICC in the ileal muscle layers in relation to the nervous system (as stained by PGP 9.5
immunoreactivity), demonstrated the absence of changes in the distribution of KIT-ir ICC in the diseased status (Figs.
The present study aims to: a) evaluate the amount and distribution of nerve fibres immunoreactive to Protein Gene Product 9.5 (PGP 9.5
), NPY and VIP, and of immunoreactive Serotonin (SER) neuroendocrine cells in several regions of the rat prostate during postnatal development; b) quantify the correlation between peptidergic innervation and the neuroendocrine cell population and its changes in postnatal development; and c) provide evidence of the value of quantitative parameters of both nerve fibres and neuroendocrine cells as relevant in distinguishing rat prostates of different ages.
Immunohistochemistry yielded a strong cytoplasmic staining for S-100 protein and moderate staining for PGP 9.5
Immunostaining for PGP 9.5
. Each section was stained with hematoxylin and eosin (H&E) for routine microscopy.
number MAB 318, Millipore, USA, working dilution 1 : 200) or PGP 9.5
(used here as panneuronal marker) (mouse, cat.