SUMF2

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SUMF2

A gene on chromosome 7q11.1 that encodes sulfatase modifying factor 2, which inhibits SUMF1-induced activation of sulfatases. It is highly expressed in the kidneys, liver and placenta.
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Then, using PCR and PFGE, we determined whether Rep.
Serological Diagnosis, PFGE Patterns, and Correlation Analysis According to the Sample Type and Serogroup
All isolates that were subjected to PFGE were first characterized with API 20E test strips (bioMerieux, Inc.
Although the DLA assay analyzes a smaller proportion of the genome and a narrower size range than PFGE and although the sequence-based mapping information is not as discriminatory as the nucleic acid sequences given by MLST, the combination of both features allows the DLA technology to possess a discriminatory power equivalent to that of PFGE and MLST.
Macrorestriction polymorphism of genomic DNA determined by PFGE revealed the presence of two pulsotypes among the nine study isolates.
All isolates of Salmonella serotype Newport and Salmonella serotype Typhimurium submitted to the Arkansas Department of Health were gathered and underwent DNA fingerprinting by PFGE using previously described techniques.
On the evolutionary history, population genetics and diversity among isolates of Salmonella Enteritidis PFGE pattern JEGX01.
Typhimurium with the matching PFGE pattern was reported, an outbreak investigation was initiated.
DNA in agarose gel plugs was digested with either SmaI (Promega) or SanDI (Stratagene) and analyzed with PFGE (CHEF Mapper; BioRad Laboratories) with 10 g/L agarose gels (SeaKem Gold; Lonza) prepared with 0.
Whereas mutational mapping found 447 polymorphisms, 12 of which disrupted genes, PFGE found only 2 differences between the Salmonella strains that varied in the ability to contaminate eggs.
To compare the discriminatory power of these approaches, the data obtained from MLST will be compared with PFGE and ribotyping for selected strains.
pneumoniae isolates (38 from ophthalmic infections, 9 from systemic infections and 14 commensals) were characterized using PFGE of the whole genome after SmaI, restriction enzyme digestion and conventional ribotyping using Escherichia coli rRNA operon as the probe.