Four phosphodiesterases (PDE4D, PDE7A, PDE7B
, and PDE8B) and two adenylate cyclases (ADCY2, ADCY4) were upregulated, and adenylate kinase (ADK) and AMP deaminase 3 (AMPD3) were downregulated in stimulated versus unstimulated cells.
Lee et al., "PDE7B
, NMBR and EPM2A variants and schizophrenia: a case-control and pharmacogenetics study," Neuropsychobiology, vol.
We also assessed the expression of PDE1 (isoforms PDE1A, 1B and 1C), and PDE10A, which hydrolyze both cAMP and cGMP, as well as the cAMP-specific PDE7 enzyme isoforms, PDE7A, and PDE7B, whose presence in the brain is well documented [34-36].
Fidock, "Cloning and characterization of the human and mouse PDE7B, a novel cAMP-Specific cyclic nucleotide phosphodiesterase," Biochemical and Biophysical Research Communications, vol.
Omori, "Identification of human PDE7B, a cAMP-specific phosphodiesterase," Biochemical and Biophysical Research Communications, vol.
Beavo, "Cloning and characterization of PDE7B, a cAMP-specific phosphodiesterase," Proceedings of the National Academy of Sciences of the United States of America, vol.
Insel, professor of pharmacology and medicine at the UC San Diego School of Medicine, has found that high levels of a enzyme, called PDE7B, in the blood are an indicator that chronic lymphocytic leukemia (CLL) - the most common form of adult leukaemia.
In the previous study, Insel's group had discovered that among a group of enzymes, cyclic nucleotide phosphodiesterases, one of the phosphodiesterases, PDE7B, was 10 times higher in CLL patients than in healthy individuals.
"The question was, could the level of PDE7B expression provide evidence for the clinical stage and diagnosis for individual patients?" Insel said.
This is a complex locus with a 10a proximal region in females containing 21 genes and a 10b distal region in both sexes containing 7 genes (Pde7b
, Ahil, Myb, HbslL, Aldh8a1, Sgkl, and Raetle) .
Specifically, the expression of ADAMTS9 (ADAM metallopeptidase with thrombospondin type 1 motif 9), HOXA11a (homeobox A11a), and PDE7B (phosphodiesterase 7B) harboring lncRNAs ADAMTS9-AS2, Hoxa11as, and NTT on the respective antisense strand was compromised, while that of ITPR1 on the opposite strand of lncRNA EGO was unaltered at both 3 or 24 h post-LPS treatment (Supplementary Table 3).
We further observed a similar expression pattern for the genes HOXA11a and PDE7B antisense to Hoxa11as and NTT lncRNAs, respectively.