The top 15 hub genes, possessing high degree of connectivity in DCM, are as follows: IL6, MYC, ACTA2, SERPINE1, ASPN, SPP1, KIT, TFRC, FMOD, PDE5A, MYH6, FPR1, C3, CDKN1A, and SOCS3.
Gene Degree of connectivity P value IL6 29 5.48E--04 MYC 17 3.99E--03 ACTA2 14 9.78E--06 SERPINE1 14 1.16E--02 ASPN 12 9.15E--03 SPP1 11 4.04E--02 KIT 11 4.54E--03 TFRC 9 3.34E--04 FMOD 9 3.42E--04 PDE5A 9 2.96E--04 MYH6 8 1.55E--03 FPR1 8 1.71E--06 C3 7 1.18E--02 CDKN1A 7 3.94E--04 SOCS3 7 1.02E--03 Table 5: Gene-specific primers used in quantitative real-time PCR.
Rosenwald et al., "t(4;8)(q27;q24) in Hodgkin lymphoma cells targets phosphodiesterase PDE5A
and homeobox gene ZHX2," Genes, Chromosomes and Cancer, vol.
The crystallographic examination of PDE5A
shows that the PDE Q pocket accommodates the pyrazolopyrimidinone group of sildenafil.
PDE5A inhibitors also can attenuate fibrosis associated PH both in patients and in the murine bleomycin model; this attenuation proceeds through the reduction of ROS and the RhoA/ROCK pathway [48, 49].
Champion, "PDE5A inhibition attenuates bleomycin-induced pulmonary fibrosis and pulmonary hypertension through inhibition of ROS generation and RhoA/Rho kinase activation," American Journal of Physiology--Lung Cellular and Molecular Physiology, vol.
Recombinant human PDE5A
was obtained from Enzo Life Sciences, Inc.
To investigate the expression of phosphodiesterases in SVZ-derived neural stem cell cultures, we assessed the presence of the cGMP-specific PDE5A
, PDE9A, and PDE6C by PCR.
MATN2, LPL, PDE5A
, CHRNA7, ROS1, BTBD9, PSMD1, CDC25A Cardiac damage lmipramine IGF1.C5 Rena!
According to Kass, RGS2 is stimulated by an enzyme, protein kinase G, whose action is, in turn, raised by countering the activity of another enzyme, phosphodiesterase 5 (PDE5A
concluded that all analyzed samples had higher levels of PDE5A
expression compared to control samples.
 found higher expression levels of PDE5A
in tumors versus normal tissues in a group of 86 PTCs and noted the presence of higher expression levels of PDE in PTCs with BRAF V600E mutation.