PCR2 | definition of PCR2 by Medical dictionary
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After qualitative and quantitative analysis, we diluted purified PCR2
products to 1 x [10.
Then, 4 [micro]L of the melted gel containing the PCR product were used for the cloning reaction, inserting the DNA fragment in the PCR2
We used DNA samples from tumors harboring 4 known FGFR3 mutations (R248C, S249C, G372C, and Y375C) to set up the PCR conditions so that only mutated DNA and not wild-type DNA was amplified in 2 different PCRs: PCR1 for detection of the R248C and G372C mutations, and PCR2
for S249C and Y375C.
(the same for both fragments), the conditions were 1X Buffer 11(Perkin-Elmer),1.