This, associated with the histochemical characteristics obtained through the AB and PAS stains
, are typical of fungi of the Cryptococcus genus (GUARNER & BRANDT, 2011; RIET-CORREA et al., 2011; GALIZA et al., 2014).
We identified information pertaining to 541 onychomycosis-associated toenail specimens sent for PAS stain
, of which 160 (29.6%) were sent by dermatologists, 198 (36.6%) by podiatrists, and 183 (33.8%) by other provider types.
Caption: FIGURE 4: (a) PAS stain
of the retinochoroidal biopsy demonstrates septate filamentous fungi with acute angle branching consistent with Aspergillus fumigatus.
The histopathology of the nail clippings sample treated with a PAS stain
demonstrates fungal elements and is the most sensitive test, but it does not identify the species.
The PAS stain
performs almost as well as GMS, in screening for fungi.
(c) Diastaseresistant eosinophilic globular inclusions, PAS stain
Caption: Figure 4: Nodular-to-diffuse, pyogranulomatous panniculitis with numerous Prototheca-Hke elements stained vividly purple with PAS stain
Visualization of the yeasts on 10% KOH preparation, Gomori's stain, PAS stain
, or Papanicolaou's smear of tissue biopsy, tracheal aspirates, BAL fluid, and sputum specimens or tissue cytological analysis or fungal culture on Sabouraud dextrose agar at room temperature are some basic methods for diagnosis of the Blastomyces dermatitidis infections.
Providing a history and the physical characteristics of the rash or a differential diagnosis will prompt the performance of a PAS stain
. Otherwise, the diagnosis can be missed because fungal elements are often not visible on routine H&E stains.
further identified ribbon like, non-septate hyphae along with developing spores (Figures 6).
The normal liver contains a large amount of glycogen, leading to hepatocytes staining intensely pink with a PAS stain
. Figure 1(a) shows that HepaRG cells stain strongly pink with PAS staining, indicative of high glycogen content, whereas HepG2 cells stain much less strongly for glycogen, with the blue nuclear counter stain dominating (Figure 1(a)).
Parenchymal staining was less intense for NS than the PAS stain
, though the vitellarium stained intensely with NS.