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The xenoestrogen, 4-nonylphenol, impaired steroidogenesis in previtellogenic oocyte culture of Atlantic cod (Gadus morhua), by targettoing the StAR protein and P450scc expressions.
2014) activity, mRNA expression level of StAR and P450scc Shah and Khan Lipid peroxidation, hydrogen peroxide, (2014) nitrite and protein levels TABLE 2.
NR0B1 (DAX1) is a transcriptional repressor of other proteins involved in the adrenal steroidogenesis pathway such as steroidogenic factor 1 (SF-1), steroidogenic acute regulatory protein (StAR), P450scc, and 3[beta]-hydroxysteroid dehydrogenase [7, 8].
Effects of a phytosterol mixture on male fish plasma lipoprotein fractions and testis P450scc activity.
Inside the mitochondria, cholesterol is converted to pregnolone by the action of P450scc enzyme (CYP11A1).
Those associated with the mitochondrial membrane are P450scc (now termed CYP11A1 (65,66)) responsible for cholesterol side-chain cleavage reaction, [P450.
Expression of the genes for 3p-hydroxysteroid dehydrogenase type 1 and cytochrome P450scc during syncytium formation by human placental cytotrophoblast cells in culture and the regulation by progesterone and estradiol.
Primer sequences which were used for the beta actin, StAR, and P450scc amplification are shown in table 1.
Screen-printed electrodes based on carbon nanotubes and cytochrome P450scc for highly sensitive cholesterol biosensors, Biosensors and Bioelectronics, 24(1), 148-150 (2008).
At the end of the incubation, the medium was collected from the 24-well plates and stored at -20[degrees]C for testosterone measurements, and cells were collected for the measurements of StAR, P450scc and 3[beta]-HSD-1 mRNA.
BPA exposure resulted in a significant downregulation of StAR expression, but P450scc and 3[beta]-HSD levels were apparently unaffected (Figure 3D,E).
Immunoreactive StAR, P450scc, or 3[beta]-HSD proteins were visualized with an avidin-biotin-enhanced horseradish peroxidase method (Vector Laboratories, Burlingame, CA, USA) using diaminobenzadine as the substrate, followed by a light nuclear counter-stain with Gill's hematoxylin.
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