We proposed the involvement of the purinergic P2Y6 receptor in this mechanism based on a strong reduction of MSU crystal-induced NET release by general purinergic receptor inhibitors and the P2Y6-specific inhibitor MRS2578 .
Reaves et al., "P2Y6 receptor antagonist MRS2578 inhibits neutrophil activation and aggregated neutrophil extracellular trap formation induced by gout-associated monosodium urate crystals," The Journal of Immunology, vol.
P2Y2 and the UDP-responsive P2Y6 receptor can also signal other chemokine productions including CXCL8 (IL-8) and CCL20 (MIP-3[alpha]) [9, 13, 14].
Joe, "Uridine 5'-diphosphate induces chemokine expression in microglia and astrocytes through activation of the P2Y6 receptor," Journal of Immunology, vol.
UTP is only known to activate P2Y2, P2Y4, and P2Y6 receptors , and, of these, the only receptor responsive to ATP is P2Y2.
(2007) UDP acting at P2Y6 receptors
is a mediator of microglial phagocytosis.
Antibodies against P2Y2, P2Y4, and P2Y6 receptors were from Alomone Labs (Jerusalem, Israel) and other antibodies were from Santa Cruz Biotech (Santa Cruz, CA, USA), from Cell Signaling (Danvers, MA, USA), or from the sources previously described .
Nasu-Tada et al., "UDP acting at P2Y6 receptors is a mediator of microglial phagocytosis," Nature, vol.