An optical trap
was generated in Zeiss inverted phase-contrast microscope by focusing an infrared laser beam (thick vertical arrow) with an objective lens (NA = 1.3).
The rotational motion is due to the torque developed by the sperm tail force acting tangentially to the force generated within the optical trap
which pins down the head of the sperm (see accompanying video in Supplementary Materials).
A typical 75-/[micro]L PCR reaction contained 200 /[micro]M each deoxynucleotide triphosphate, 1.0 U of polymerase, 20 pmol of each primer, reaction buffer (containing [MgCl.sub.],) supplied with the polymerase, and either HeLa genomic DNA (100 ng) or a single HeLa cell isolated from an optical trap
. PCR conditions were 95 [degrees]C for 45 s (2 min on first cycle), 57-62 [degrees]C for 45 s, and 72 [degrees]C for 1.5 min for 25-50 cycles on a PTC-100 thermal cycler (MJ Research).
A single-beam gradient-force optical trap
, called an optical tweezers, uses a microscope objective lens to focus a single beam of laser light.
Using the newly developed light-scanning optical trap
, however, the biophysicists were able to hold and orient the bacteria over their entire length.
The minimum power at which the microsphere was pulled into the optical trap
Chu, "Observation of a single-beam gradient force optical trap
for dielectric particles," Opt.
The new record is of 7 milliseconds for rubidium atoms stored in a dipole optical trap
, while the previous record for storage time was 32 microseconds- a difference of more than two orders of magnitude.
The team applied a gradient-force optical trap
, or "optical tweezers," to mitochondria cruising along microtubules within an amoeba.
Tenders are invited for purchase of two (1) model trap7-si-c-bnc-d0 7mm, silicon optical trap
collimated beams quote eo18_2439-1, (2) two trap-preamp low noise high gain amplifier with digital readout quote eo18_2439-1.
These probes will be held at a fixed position within the matrix using an optical trap
and their Brownian motion in all three spatial dimensions tracked using multiplane imaging.
"The advantage of the optical trap
is that you can sort cells with absolute purity," since the monitor allows the operator to inspect each cell visually, Buican says.