Duck hepatitis B virus expresses a regulatory HBx-like protein from a hidden open reading frame
The expression vector of recombinant SPLUNC1 protein was constructed by inserting full length SPLUNC1 open reading frame
(ORF) into pPIC9K plasmid (Invitrogen, China).
Heteroplasmy can be benign, but because of the small size and high density of open reading frames
for essential genes within the mitochondrial genome, sequence inconsistencies can prove fatal.
Each splice variant is then further annotated with information about homologues, SAGE-based expression profiles, open reading frames
, sequence translation and detailed domain summaries.
In support of this open technology platform, Life Technologies has established a series of collaborations, the focus of which is to provide the scientific community broad access and maximal utility to clones and full-length open reading frames
enabled with Gateway Technology.
The fact that most of the 5'-proximal AUG sequences examined were blocked by downstream stop codons is a striking coincidence and raises the further question of what the functions of short, upstream open reading frames
might be aside from the possibility that they encode short polypeptides with as yet undiscovered biological functions.
Invitrogen has utilized the power of Gateway(R) Technology in building its Ultimate(TM) ORF Clone Collection -- a wide-ranging collection of full-insert sequenced human and mouse open reading frames
Nucleotide sequencing combined with a search of the GenBank database revealed five clones generated by 5'RACE-PCR ([approximately equal to] 500) (Figure 1), as having no the initiator element in their open reading frames
However, the team has noted that expression of some of these viral open reading frames
has been detected in human tissues, which supports the possibility that they are biologically active in host species.
Sequence analysis indicated that the tentatively named raven circovirus (RaCV) was 1898 nucleotides in size with 2 major open reading frames
synonymous with other avian circoviruses, ORF C1 and ORF V1, likely to encode a putative capsid protein (Cap) and replicase-associated protein (Rep), respectively.
In some cases, earlier research may have revealed the locations and sequences of specific genes, but for large-scale identification of potential genes, scientists run computer programs to find stretches of DNA known as open reading frames
Isolated pieces of RNA are labeled with a fluorescent dye and then allowed to hybridize to chips, each of which is studded with up to 40 oligonucleotides complementary to each of the 6,200 specific yeast genes and open reading frames
(areas that are likely to be protein-coding regions of the genome).