We requested microscopic analysis (with an oil immersion objective
lens at 1,000x) of earlobe skin samples.
Measurements were taken from 30 oocysts under an oil immersion objective
(Duszynski and Wilber, 1997) within 6 wk after they had completed sporulation, and these are reported in micrometers ([micro]m) with means followed by the ranges in parentheses.
Tape impression smears collected from ears revealed budding yeast cells under oil immersion objective
after staining with new methylene blue (Fig.
Even in moderately preserved specimens the taenae were partly or totally corroded and could only be identified by their faint outline detectable under oil immersion objective
Gram's Stain: A drop of uncentrifuged, well mixed urine was taken on a clean grease-free slide and stained by Gram's staining and examined under the oil immersion objective
of the microscope (examining 20 fields).
He also talks of his "disastrous" year in neuropathology and neurochemistry: "I screwed the oil immersion objective
of the microscope through several priceless slides.
"Resolution of morphologic detail adequate to permit reasonably reliable identification of parasites and discrimination among parasite species requires use of a 100X oil immersion objective
," says Shapiro, who remains unconvinced about how well the new technology can reliably identify the presence of malaria, if at all.
The smears were graded using 100 x oil immersion objective
as per RNTCP guidelines (1): Scanty = 1- 9 AFB in 100 oil immersion fields (field); 1 + = 10 - 99 AFB in 100 fields; 2+ = 1 to 9 AFB per field in at least 50 fields; 3+ = 10 or more AFB per field in at least 20 fields; Neg = no AFB in 100 fields.
Carl Zeiss Jena produced its first oil immersion objective
in 1880, designed by Ernst Abbe, who was the founder of the optical theory of microscope lenses.
* The oil immersion objective
lens is rotated into viewing position (because I want to see the student switch to the scanning or 10X objective lens, focus, and then move to a higher power objective).