The medicinal plants such as, Acalypha indica, Leucas aspera, Ocimum tenuiflorum L and Solanum nigrum were selected in and around Thanjavur, Tamil Nadu, India.
Total heterotrophic bacteria, fungi, and Micromonospora species were enumerated from the rhizosphere and non-rhizosphere soil samples of 4 selected medicinal plants like Acalypha indica, (Kupaimani), Leucas aspera (Dumbi), Ocimum tenuiflorum L (Tulasi) and Solanum nigrum (Manathakali).
The gram staining technique was carried out for the bacterial colonies isolated from 4 different medicinal plants (Acalypha indica, Leucas aspera, Ocimum tenuiflorum L and Solanum nigrum).
Gram negative rod shaped bacterial colonies from Leucas aspera root sample and soil sample and from Ocimum tenuiflorum L root sample were stabbed and streaked in the oxidative fermentative medium and the change of pink to yellow color was observed for indication of the acid production.
The varying degrees of population observed in the roots of the plants is due to the effect of the chemical composition of root exudates of the individual plants such as Acalypha indica, Leucas aspera, Ocimum tenuiflorum L and Solanum nigrum on the microorganism.
On the other hand, a combination of the plants--Terminalia belerica, Terminalia chebula, Ocimum sanctum, Ocimum tenuiflorum
, and Terminalia arjuna was used for treatment of a single affliction, namely pain in bones.