Then correlations between OLR1 and other genes were analyzed.
Cloning and sequencing of OLR1 gene in pig adipose tissue
We extracted total RNA from adipose tissue of GB pigs and amplified OLR1 cDNA complete CDS by RT-PCR, and the total RNA and amplification products were detected by agarose gel electrophoresis (displayed in Figure 1).
After removal of residues in N and C terminal nontranslating regions by BLAST alignment to the sequencing analysis, we obtained pig adipose tissue OLR1 gene CDS which contained 825 bp and its accession No.
Analysis of OLR1 gene sequence in pig adipose tissue
We aligned the sequence of OLR1 gene CDS in pig adipose tissue with that of related species published in GenBank and found that the OLR1 gene in pig adipose tissue exhibited 99% homology to that in pig aortic endothelial tissue (NM_213805).
The analysis for physical chemistry parameters of OLR1 protein revealed that OLR1 had a molecular weight of 31,197.
We predicted a signal peptide for pig adipose tissue OLR1 protein and the result is displayed in Figure 2.
The analysis for domains and conserved domain of pig OLR1 protein is presented in Figure 3.
The expression abundance of OLR1 gene mRNA in different pig tissues
Amplification of a 200 bp fragment of the OLR1 gene in different pig tissues (Figure 4) indicated that the highest relative expression (compared to [beta]-actin) was in the lung and the lowest was in the spleen and visceral adipose tissue, whereas expression in other tissues was at intermediate levels.
The expression characteristic of OLR1 in adipose tissue at different MA and in different economic types of pigs