pCAF Associates with CIITA Acetylation Null Mutants
. As pCAF acts in a dual way as both HAT and ubiquitin ligase, and as acetylation drives CIITA nuclear import, we next determined if acetylation of CIITA is necessary for CIITA ubiquitination mediated by pCAF.
CBP1 null mutants
(CBP1 [delta]) are unable to induce macrophage apoptosis and are avirulent in murine model of pulmonary infection [45,46].
To determine whether CK1.1 is essential for parasite survival, we generated a CK1.1 null mutant
Fgfr1 null mutant
embryos die during gastrulation and segmentation, while homozygous embryos of Fgfr2 knockout die before gonad formation (18, 19).
Dopamine and benzodiazepine-dependent mechanisms regulate the EtOH-enhanced locomotor stimulation in the GABAA alpha1 subunit null mutant
Residual guanosine 3', 5'-bispyrophosphate synthetic activity of relA null mutants
can be eliminated by spoT null mutations.
To make null mutant
mice, it takes at least about one year from the construction of the targeting vector if there is no delay during the whole procedure from steps such as colony selection of ES cells, chimera production and confirmation of germ line transmission for the mice.
Decreased ethanol sensitivity and tolerance development in gamma-PKC null mutant
mice is dependent on genetic background.
1987); and the null mutant
[Adh.sup.n1], which has an inactive alcohol dehydrogenase (ADH) enzyme, was used for in vivo experiments (Grell et al.
In contrast, a loss-of-function mutant of SCM3, fc1, and another SL synthesis null mutant
, d10, (42) exhibited not only desirable SL-related phenotypes (increased tiller number) but also unfavorable reduction in culm diameter and thickness.
lin-11 null mutant
animals display cryophilic defects that phenocopy laser ablation of the AIZ interneuron.
(68) gon-1 null mutants
show a number of severe phenotypes, including sterility, but we also found that certain genes, if downregulated, suppress even such severe phenotypes (Yoshina et al., in preparation).