anneal

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Related to Nucleic acid hybridization: nucleic acid probe

anneal

 [ah-nēl´]
1. to heat a material, such as glass or metal, followed by controlled cooling to remove internal stresses and induce a desired degree of toughness, temper, or softness of the material.
2. to homogenize an amalgam alloy ingot by heating it in an oven.
3. to heat a material, such as gold foil, to volatilize and drive off impurities from its surface, and to increase its cohesive properties.

an·neal

(an-nēl'),
1. To soften or temper a metal by controlled heating and cooling; the process makes a metal more easily adapted, bent, or swaged, and often less brittle.
2. In dentistry, to heat gold leaf preparatory to its insertion into a cavity, to remove adsorbed gases and other contaminants.
3. The pairing of complementary single strands of DNA; or of DNA-RNA.
4. The attachment of the ends of two macromolecules, for example, two microtubules annealing to form one longer microtubule.
5. In molecular biology, annealing is a process in which short sections of single-stranded DNA from one source are bound to a filter and incubated with single-stranded, radioactively conjugated DNA from a second source. Where the two sets of DNA possess complementary sequences of nucleotides, bonding occurs. The degree of relatedness (homology) of the two sets of DNA is then estimated according to the radioactivity level of the filter. This technique plays a central role in the classification of bacteria and viruses. Synonym(s): nucleic acid hybridization
6. To renature proteins or polynucleic acids by slow cooling.
[A.S. anaelan, to burn]

anneal

/an·neal/ (ah-nēl´)
1. to toughen, temper, or soften a material, as a metal, by controlled heating and cooling.
2. in molecular biology, to cause the association or reassociation of single-stranded nucleic acids so that double-stranded molecules are formed, often by heating and cooling.

anneal

Etymology: AS, aelan, to burn
1 to temper metals, glass, or other materials by controlled heating and cooling to make them more malleable and ductile.
2 to cause the interaction of two separate strands of nucleic acid to form a duplex molecule, often by using a related technique of controlled heating and cooling.

an·neal

(ă-nēl')
Process by which oligonucleotides affix to targeted DNA sequences.
[A.S. anaelan, to burn]

an·neal

(ă-nēl')
In dentistry, to heat gold leaf preparatory to its insertion into a cavity to remove adsorbed gases and other contaminants.
[A.S. anaelan, to burn]

anneal (anēl´),

n (homogenizing heat treatment, softening heat treatment), the softening of a metal by controlled heating and cooling.
anneal foil,
n a process of subjecting noncohesive foil to heat to volatilize a protective gaseous coating on its surface, thus leaving the surface clean, making it cohesive.
anneal glass,
n a process of regulated heating and subsequent cooling to remove strain hardening or work hardening of glass.
anneal metal,
n a process of regulated heating and subsequent cooling to remove strain hardening or work hardening of metal.
References in periodicals archive ?
Immunoassays [6], nucleic acid hybridization [7], nucleic acid amplification [5, 8-10], lux phage assay [11, 12] and combined cultural-molecular methods [13] are the examples.
The flow cell is aligned to adhere to the glass slide to form a nucleic acid hybridization unit.
Methods for nucleic acid hybridization analysis using stem-loop DNA "hairpin" probes, or an array of immobilized probes are also protected.
The inventions claimed in this patent are directed to specific advantages that the technology provides for detecting nucleic acid hybridization, namely detection of hybridization without requiring the separation of unbound from bound nucleic acids, thereby speeding up the detection of hybridization.
Signature's MCS technology uses radio wave and micro-wave frequencies to detect the physical and biological properties of molecules and cells, such as target drug receptors, and to detect the interaction of two molecules with each other in real time, such as the binding of a test compound to a target drug receptor or the formation of a nucleic acid hybridization complex.
Both tests are qualitative in vitro tests for the direct detection of Hepatitis C virus RNA and Human Immunodeficiency virus (HIV-1) RNA in human plasma using PCR nucleic acid amplification and nucleic acid hybridization.
Simultaneously, the use of assays based on nucleic acid hybridization has increased rapidly, especially given the availability of effective amplification technologies such as PCR [4,5].
announced today that the Australian Patent Office has granted patent number 724,600, entitled, "Electrochemical detection of nucleic acid hybridization.
The first three review the basic principles of nucleic acid hybridization, probe generation, and probe labeling; the remaining chapters review applications of these techniques in genetic screening, forensics, infectious diseases, human medicine, and food and environmental sciences.
MicroDenteX holds an exclusive license for the use of DNA testing for periodontal disease by nucleic acid hybridization.
Factors that could cause actual results to differ from those projected include the possibility that, due to difficulties in the development of the technology, BBI may not be successful in developing pressure cycling technology into commercially viable products or services, including those in the area of sterilization and nucleic acid hybridization and amplification; that pressure cycling technology may not be adaptable to any other commercially viable applications; that certain pressure cycling applications may not fall within the claims of the Company's eight issued patents; and that individuals and groups utilizing such PCT procedures may not be required to license such technology from BBI.