anneal

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Related to Nucleic acid hybridization: nucleic acid probe

anneal

 [ah-nēl´]
1. to heat a material, such as glass or metal, followed by controlled cooling to remove internal stresses and induce a desired degree of toughness, temper, or softness of the material.
2. to homogenize an amalgam alloy ingot by heating it in an oven.
3. to heat a material, such as gold foil, to volatilize and drive off impurities from its surface, and to increase its cohesive properties.

an·neal

(an-nēl'),
1. To soften or temper a metal by controlled heating and cooling; the process makes a metal more easily adapted, bent, or swaged, and often less brittle.
2. In dentistry, to heat gold leaf preparatory to its insertion into a cavity, to remove adsorbed gases and other contaminants.
3. The pairing of complementary single strands of DNA; or of DNA-RNA.
4. The attachment of the ends of two macromolecules, for example, two microtubules annealing to form one longer microtubule.
5. In molecular biology, annealing is a process in which short sections of single-stranded DNA from one source are bound to a filter and incubated with single-stranded, radioactively conjugated DNA from a second source. Where the two sets of DNA possess complementary sequences of nucleotides, bonding occurs. The degree of relatedness (homology) of the two sets of DNA is then estimated according to the radioactivity level of the filter. This technique plays a central role in the classification of bacteria and viruses. Synonym(s): nucleic acid hybridization
6. To renature proteins or polynucleic acids by slow cooling.
[A.S. anaelan, to burn]

an·neal

(ă-nēl')
Process by which oligonucleotides affix to targeted DNA sequences.
[A.S. anaelan, to burn]

an·neal

(ă-nēl')
In dentistry, to heat gold leaf preparatory to its insertion into a cavity to remove adsorbed gases and other contaminants.
[A.S. anaelan, to burn]
References in periodicals archive ?
The medical technicians' diagnoses served as the criterion standard for comparison with the clinician evaluations and nucleic acid hybridization test results.
Following successful completion of a preinvestigation proficiency test, a medical technician processed the nucleic acid hybridization tests (Affirm VP III, Micro-Probe Probe Corporation, Bothell, Wash) submitted during the study.
Microfluidic chip for fast nucleic acid hybridization. Lab Chip 2003;3:228-33.
Devices and methods for optical detection of nucleic acid hybridization. US patent no.
Direct detection of nucleic acid hybridization on the surface of a charge coupled device.
Nucleic acid hybridization is commonly analyzed by colorimetric, chemiluminescent, radioactive, or fluorescent label detection (1).
Simultaneously, the use of assays based on nucleic acid hybridization has increased rapidly, especially given the availability of effective amplification technologies such as PCR [4,5].
As examples of the assays that can be performed with the Hartman interferometer, we show direct immunoassays for human chorionic gonadotropin (hCG) (3), influenza A virus, and Salmonella typhimurium and direct nucleic acid hybridization measurements preliminary to the development of an assay for the differentiation of Mycobacterium species.
The first three review the basic principles of nucleic acid hybridization, probe generation, and probe labeling; the remaining chapters review applications of these techniques in genetic screening, forensics, infectious diseases, human medicine, and food and environmental sciences.