Northern blotting


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A technique used to detect a specific mRNA sequence, which consists of electrophoresing RNA fragments, transferring those fragments to a membrane, then adding a labelled nucleic acid probe to the milieu
Segen's Medical Dictionary. © 2012 Farlex, Inc. All rights reserved.

Northern blotting

Molecular biology A technique used to detect the presence of a specific mRNA sequence. See Blotting, Hybridization, Probe, RNA, Southern blotting.
McGraw-Hill Concise Dictionary of Modern Medicine. © 2002 by The McGraw-Hill Companies, Inc.

Northern transfer

or

Northern blotting

a method for transferring BANDS of RNA, separated by GEL ELECTROPHORESIS, from the electrophoresis gel to a membrane support. The method is named by analogy with SOUTHERN TRANSFER.
Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005
References in periodicals archive ?
In preliminary control experiments, when Northern blotting was carried out with individual probes alone, using identical hybridization and wash conditions, the hybridization patterns showed single bands of appropriate sizes for each probe (18S rRNA, 2000 nt; FAMTase mRNA, 1250 nt).
Applications include gene expression, single nucleotide polymorphisms analysis, genotyping, drug screening, in situ tissue analysis, immunohistochemical analysis, cellular receptor binding detection, Western and Northern blotting, flow cytometry, microfluidics, microarrays, and multiple clinical diagnostic applications.
In the other,[24] failure to detect c-kit mRNA by Northern blotting could have resulted owing to the analysis of total RNA rather than poly-A-enriched RNA.
When checked by a number of methods including quantitative RT-PCR (6, 35), Northern blotting (33, 34, 36), and protein expression (33, 34), most differentially expressed genes have been confirmed.
There has been one previously published slot blot/RPA protocol, but this method differs in that it preserves the basic advantages of RPA over northern blotting; i.e., greater sample capacity and hybridization efficiency in solution.
These markers are used by researchers and companies for various applications such as polymerase chain reaction (PCR), northern blotting, southern blotting, and molecular cloning.
LPL mRNA levels were determined by Northern blotting and laser scanning of autoradiographs.
It is a more sensitive method to quantify gene expression than conventional methods used for mRNA evaluation, such as Northern blotting, dot-blot analysis, and the RNase protection assay.
It is classified into PCR, sequencing, northern blotting, southern blotting, molecular cloning, and other nucleic acid applications.
We isolated the full-length cDNA of porcine FoxO1 gene using RACE, confirmed by visual Northern blotting. The deduced amino acids indicated 94% and 90% identities with the corresponding human and mice aa.
The protein and mRNA contents of UGT2B1 also were not detected in the fetal rat liver, but a developmental increase in newborn rat liver was detected by Western and Northern blotting analysis.

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