In preliminary control experiments, when Northern blotting
was carried out with individual probes alone, using identical hybridization and wash conditions, the hybridization patterns showed single bands of appropriate sizes for each probe (18S rRNA, 2000 nt; FAMTase mRNA, 1250 nt).
Applications include gene expression, single nucleotide polymorphisms analysis, genotyping, drug screening, in situ tissue analysis, immunohistochemical analysis, cellular receptor binding detection, Western and Northern blotting
, flow cytometry, microfluidics, microarrays, and multiple clinical diagnostic applications.
In the other, failure to detect c-kit mRNA by Northern blotting
could have resulted owing to the analysis of total RNA rather than poly-A-enriched RNA.
When checked by a number of methods including quantitative RT-PCR (6, 35), Northern blotting
(33, 34, 36), and protein expression (33, 34), most differentially expressed genes have been confirmed.
There has been one previously published slot blot/RPA protocol, but this method differs in that it preserves the basic advantages of RPA over northern blotting
; i.e., greater sample capacity and hybridization efficiency in solution.
These markers are used by researchers and companies for various applications such as polymerase chain reaction (PCR), northern blotting
, southern blotting, and molecular cloning.
LPL mRNA levels were determined by Northern blotting
and laser scanning of autoradiographs.
It is a more sensitive method to quantify gene expression than conventional methods used for mRNA evaluation, such as Northern blotting
, dot-blot analysis, and the RNase protection assay.
. Total RNA and protein was extracted from hypothalami using the Trizol reagent (Gibco/BRL).
It is classified into PCR, sequencing, northern blotting
, southern blotting, molecular cloning, and other nucleic acid applications.
We isolated the full-length cDNA of porcine FoxO1 gene using RACE, confirmed by visual Northern blotting
. The deduced amino acids indicated 94% and 90% identities with the corresponding human and mice aa.
The protein and mRNA contents of UGT2B1 also were not detected in the fetal rat liver, but a developmental increase in newborn rat liver was detected by Western and Northern blotting