In preliminary control experiments, when Northern blotting was carried out with individual probes alone, using identical hybridization and wash conditions, the hybridization patterns showed single bands of appropriate sizes for each probe (18S rRNA, 2000 nt; FAMTase mRNA, 1250 nt).
To determine the tissue distribution and developmental expression of the putative FAMTase, Northern blotting was carried out using the 450-bp clone as a probe.
This retains the advantages of RPA over northern blotting
, which is its greater sample capacity and hybridization efficiency by performing the digestion in solution rather than on the membrane.
LPL mRNA levels were determined by Northern blotting
and laser scanning of autoradiographs.
It is a more sensitive method to quantify gene expression than conventional methods used for mRNA evaluation, such as Northern blotting
, dot-blot analysis, and the RNase protection assay.
We isolated the full-length cDNA of porcine FoxO1 gene using RACE, confirmed by visual Northern blotting
The protein and mRNA contents of UGT2B1 also were not detected in the fetal rat liver, but a developmental increase in newborn rat liver was detected by Western and Northern blotting
Neither genomic Southern blotting nor Northern blotting
of tumor RNA can be performed successfully with the degraded nucleic acids obtained from fixed and embedded tissue specimens.
To estimate the expression sites of the two genes in the foot, RNA was isolated separately from the distal end and from the remainder of the foot and was also analyzed by northern blotting
We next examined the effects of cadmium on the transcript level of bcl-2 by Northern blotting
, comparing with that of c-myc as the immediate early gene.
Although duplicate performance of experiments may seem time consuming, our experience has shown that the extra effort expended at this stage is more than repaid by reducing unnecessary cloning, sequencing, and Northern blotting
later in the process.