The simplest and most readily visualized of these methods hail from the early days of molecular biology: Southern and Northern blots
Although Southern and Northern blots
illustrate important principles about nucleic acid chemistry and hybridization, they are very difficult to describe in lecture: it is an elaborate process to separate nucleic acid molecules by size, move the nucleic acid from gel to blot, radioactively label a probe, hybridize the probe to a target sequence on the blot, and detect the hybridized probe by exposure of the blot to x-ray film.
That the invention of Western blotting was not blindingly intuitive to someone like me, who was performing Southern and Northern blots
(1, 2) on a regular basis, perhaps seems incomprehensible now, but struggle I did.
In addition, densitometry of Northern blots
also showed a significant depot differences in rats (Table 2).
5 kb was detected in Northern blots
containing samples from TSV challenges shrimp.
While the labeling in the PlatinumBright kits have been designed to provide the optimal labeling density for Fluorescent in situ hybridization (FISH) applications, the ULS labeling reaction in these kits are also optimal for filter hybridizations such as Northern Blots
, Southern Blots, Spot Blots and Dot Blot hybridizations as well as use with Tissue Arrays and ChIP on Chip experiments (labeling of promoter fragments after Chromatin immuno precipitation).
The authors used Northern blots
to compare the amount of mRNA produced in exposed versus control cells.
Analyses utilizing Northern blots
and polyacrylamide gel electrophoresis revealed that all six Sry copies are potentially active.
This ensures that the materials are suitable for all forms of gene expression analysis - from Northern blots
, to TaqMan to Affymetrix chips.
The transcript of FoxO1 was detected by virtual Northern blots
To determine if the gene expression profiles on the array could be verified by other techniques that monitor mRNA expression, we compared the expression profiles of several genes on the arrays (Vtg 2, choriogenin 2, and transferrin) to their profile by Northern blots
and DD RT-PCR.
for three gene transcripts, GAPDH, IFN IEF SSP [an interferon (IFN)-[gamma]-activated gene], and p53, were performed on blood stored in either PAXgene or EDTA tubes at 22[degrees]C for 7 days.