Sensitive and specific detection of microRNAs by northern blots
analysis using LNA- modified oligonucleotide probes.
The simplest and most readily visualized of these methods hail from the early days of molecular biology: Southern and Northern blots
However, on the Northern blots
performed for miRNAs 171b&c, 447c and 399dwe never detected mature forms (data not shown).
Although Southern and Northern blots
illustrate important principles about nucleic acid chemistry and hybridization, they are very difficult to describe in lecture: it is an elaborate process to separate nucleic acid molecules by size, move the nucleic acid from gel to blot, radioactively label a probe, hybridize the probe to a target sequence on the blot, and detect the hybridized probe by exposure of the blot to x-ray film.
That the invention of Western blotting was not blindingly intuitive to someone like me, who was performing Southern and Northern blots
(1, 2) on a regular basis, perhaps seems incomprehensible now, but struggle I did.
The Big Shot III hybridization oven is suited for all types of hybridization and incubation applications, including northern blots
, southern blots and microarrays.
In addition, densitometry of Northern blots
also showed a significant depot differences in rats (Table 2).
5 kb was detected in Northern blots
containing samples from TSV challenges shrimp.
Assay for transcriptional expression of the HVA1 transgene was performed by Northern blots
The authors used Northern blots
to compare the amount of mRNA produced in exposed versus control cells.
Analyses utilizing Northern blots
and polyacrylamide gel electrophoresis revealed that all six Sry copies are potentially active.
were used to determine the tissue distributions and developmental appearances of these mRNAs.