Northern blot analysis


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North·ern blot a·nal·y·sis

a procedure similar to the Southern blot analysis, used to separate and identify RNA fragments; typically through transferring (blotting) RNA fragments from an agarose gel to a nitrocellulose filter followed by detection with a suitable probe.
[coined to distinguish it from eponymic Southern blot a.]
Farlex Partner Medical Dictionary © Farlex 2012

Northern blot analysis

n.
A technique for identifying specific sequences of RNA in which RNA molecules are separated by electrophoresis, transferred to nitrocellulose, and identified with a suitable probe.
The American Heritage® Medical Dictionary Copyright © 2007, 2004 by Houghton Mifflin Company. Published by Houghton Mifflin Company. All rights reserved.

North·ern blot a·nal·y·sis

(nōr'dhĕrn blot ă-nal'i-sis)
A procedure similar to the Southern blot analysis, used mostly to separate and identify RNA fragments; typically done through transferring RNA fragments from an agarose gel to a nitrocellulose filter followed by detection with a suitable probe.
[coined to distinguish it from eponymic Southern blot a.]
Medical Dictionary for the Health Professions and Nursing © Farlex 2012
References in periodicals archive ?
Northern blot analysis of the p53 gene showed that, in PAXgene tubes, the mRNA was preserved for 7 days at room temperature, although the intensity of the transcript band diminished slightly at 5 and 7 days of storage.
Only a select number of genes were found to be up-regulated by HDI using microarray analysis, and these effects were confirmed using Northern blot analysis. Although there has been rapidly growing interest in microarray technology, this is one of the first reports demonstrating its utility in studying the effects of exposure to occupationally relevant chemicals.
The Northern blot analysis demonstrated the occurrence of a 1.4-kb long CiEnds3 transcript, which was detected only in the endostyle [ILLUSTRATION FOR FIGURE 4C OMITTED].
In addition, the Northern blot analysis demonstrated the occurrence of a 1.6-kb-long CiEnds4 transcript not only in the endostyle but also in other organs including the pharyngeal gill, body-wall muscle, intestine, and gonad [ILLUSTRATION FOR FIGURE 5B OMITTED].
Total RNA samples from hepatoma cell lines containing various amounts of 2-5AS mRNA were analyzed by C-PCR and Northern blot analysis, and the results were compared (Fig.
Linear and reproducible quantification was confirmed by the reconstitution experiment, correlating the results with those obtained by Northern blot analysis. To the best of our knowledge, this is the first report demonstrating the quantification of distinct forms of 2-5AS mRNA by C-PCR.
The findings presented in this paper indicate that RNA isolated by cesium chloride ultracentrifugation is optimally suited for a wide range of molecular applications, including northern blot analysis, in vitro translation, and cDNA synthesis from dying tissues of Botryllus schlosseri and other colonial ascidians.
Northern blot analysis with cesium-chloride-purified material further revealed that the RNA was not degraded by ribonuclease activity present during zooid regression.
6 shows the Northern blot analysis of ABCA1 mRNA in macrophages from three healthy donors (Fig.
As we demonstrated previously by Northern blot analysis (4) and semiquantitative RT-PCR (28), expression of human ABCA1 mRNA in macrophages is regulated in a differentiation-dependent fashion.
Moreover, Northern blot analysis also revealed that it did not affect P450scc mRNA levels (Figure 5).
To determine if Roundup reduced StAR protein levels by decreasing StAR mRNA levels, we performed Northern blot analysis (Figure 7).

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