Northern blot


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Northern blot

an immunologic technique for the detection of specific messenger RNAs using complementary DNA. Called also Northern transfer.
References in periodicals archive ?
Southern blot and Northern blot hybridizations were performed as described previously by Lee et al.
En diferentes estudios de la expresion mediante Northern blot y de hibridacion in situ, diversos genes de expansinas son expresados en diferentes organos, tejidos y tipos de celula y responden distinto a tratamientos hormonales de luz y polinizacion, asi como la programacion endogena de desarrollo, mostrando que las expansinas estan implicadas en una gran variedad de respuestas al desarrollo y crecimiento, desde germinacion y maduracion de frutos hasta polinizacion (Cosgrove et al.
To validate array results we performed Northern blot hybridizations and RT PCR.
Tissue-specific cPdcl2 expression was examined in the brain, liver, muscle, spleen, kidney, testis, and ovary of 24-week-old chickens by Northern blot using a digoxigenin Northern Starter Kit (Roche; Holtke et al.
For Northern blot analysis, l0[micro]g of total RNA of each sample was denatured by incubation for 1 h at 50 [degrees]C in a solution of 0.
For the Northern blot, 20 [micro]g of RNA were separated in a 1.
We obtained a human 12-tissue, multiple tissue, Northern blot from Clontech and probed this with recombinant probes corresponding to the putative OPmV M and P genes.
Following densitometric analysis of the Northern blot autoradiograms, the ratio of FAMTase to 18S rRNA was determined, and the results were displayed graphically (Fig.
Finally, 10 of these pairs were further analyzed using northern blot analysis with strand-specific riboprobes.
Total RNA were extracted from the treated cells and Northern blot or ribonuclease protection assays were performed, these experiments showed that the expression level of some of the ADAMs are modulated by growth factor treatment.
6] The sodium/iodide symporter outside the thyroid gland, however, does not appear to be affected by thyroidstimulating hormone, even though the sequence appears identical to that of the thyroid receptor on Northern blot analysis and DNA sequencing analysis.

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