Northern blot analysis

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North·ern blot a·nal·y·sis

a procedure similar to the Southern blot analysis, used to separate and identify RNA fragments; typically through transferring (blotting) RNA fragments from an agarose gel to a nitrocellulose filter followed by detection with a suitable probe.
[coined to distinguish it from eponymic Southern blot a.]
Farlex Partner Medical Dictionary © Farlex 2012

Northern blot analysis

n.
A technique for identifying specific sequences of RNA in which RNA molecules are separated by electrophoresis, transferred to nitrocellulose, and identified with a suitable probe.
The American Heritage® Medical Dictionary Copyright © 2007, 2004 by Houghton Mifflin Company. Published by Houghton Mifflin Company. All rights reserved.

North·ern blot a·nal·y·sis

(nōr'dhĕrn blot ă-nal'i-sis)
A procedure similar to the Southern blot analysis, used mostly to separate and identify RNA fragments; typically done through transferring RNA fragments from an agarose gel to a nitrocellulose filter followed by detection with a suitable probe.
[coined to distinguish it from eponymic Southern blot a.]
Medical Dictionary for the Health Professions and Nursing © Farlex 2012
References in periodicals archive ?
Indeed, the northern blot data shown in Figure 2B demonstrated that the Enpp1 mRNA level was either slightly reduced or increased in primary hepatocytes treated with 100 nM insulin or 100 nM dexamethasone for 12 h, respectively.
Over expression of MsHsp23 transgenic alfalfa plants: We confirmed the expression of four lines (Tg1, Tg3, Tg4 and Tg7) by Northern blot analysis.
The expression of DR6 mRNA was measured by Northern blot at different time points as indicated.
Since binding/internalization of the aptamer could only reliably be detected by Northern blot, we did not screen many other cell lines for binding.
Small interfering RNAs (siRNAs) 21-24 nts in size, corresponding to the CP:Hc-Pro sequence of the Ubi-hpCP:HcPro, were readily detected by Northern blot analysis in all the five independent lines (Figure 4(a)), indicating that the Ubi-hpCP:Hc-Pro was expressed and the resulting hpRNA processed by Dicer (DCLs) enzyme which recognizes and cleaves the dsRNA into siRNAs.
The expression of PHYB transgene was analyzed by Northern blot analysis (Figure 3(c)).
We extracted RNAs from 12 different organs and analysed by northern blot the amount of siRNA produced in hemizygous animals.
To validate array results we performed Northern blot hybridizations and RT PCR.
Tissue-specific cPdcl2 expression was examined in the brain, liver, muscle, spleen, kidney, testis, and ovary of 24-week-old chickens by Northern blot using a digoxigenin Northern Starter Kit (Roche; Holtke et al., 1995).
Northern blot analysis has shown that M46 is expressed in G186AS and Downs strains, but is transcriptionally silent in G184AS and G217B strains.
Femoral mRNA levels of selected bone matrix proteins were determined by northern blot analysis.

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