Northern blot


Also found in: Dictionary, Encyclopedia, Wikipedia.

Northern blot

an immunologic technique for the detection of specific messenger RNAs using complementary DNA. Called also Northern transfer.
References in periodicals archive ?
Because the relative expression levels of cPdcl2 were high in the testis of 24-weeks-old chickens, we further examined the specificity of cPdcl2 in several tissues of 24-week-old chickens using Northern blot.
Northern blot analysis of total RNA from planktonic and biofilm-grown cells demonstrated that ALS gene expression is differentially regulated between the two growth forms, with additional genes expressed in biofilms (4,5).
The transcript of FoxO1 was detected by virtual Northern blots.
Constant volumes, corresponding to the volumes of the to samples, were loaded on the gels for the Northern blot analysis to investigate the general stability of mRNA in blood samples.
Northern blot analysis using human LDHA cDNA as a probe showed that R51 cells did not express wild-type LDHA mRNA but did express a small amount of abnormal mRNA with a molecular mass slightly higher than that of wild-type LDHA (Fig.
To validate microarray results and to further study the kinetics of HDI-induced changes in gene expression patterns, we undertook Northern blot analyses.
Appropriate clones were selected, grown in LB medium with kanamycin, purified, and sequenced as described above to serve as DNA competitors to be used in C-PCR or as cDNA probes for Northern blot analysis as described below (Table 2).
We have yet to determine if the two other mRNAs identified by Northern blot analysis arise from distinct p53 homologue genes or are shortened versions of p120.
Furthermore, high concentrations of TIMP-1 mRNA, analyzed using the Northern blot method, have been associated with metastasis in breast cancer (32).

Full browser ?