More recently, quantitative reverse transcription PCR, Northern analysis
, and in situ hybridization have been adapted to quantify miRNAs.
The genes selected by differential screening were subjected to Northern analysis to confirm the differential expression of genes between the two genetic breeds of chickens.
Two genes were confirmed by Northern analysis to be differentially expressed in the livers of different lines of chickens.
Northern analysis and Triton X-114 soluble protein tests were performed on seeds from [F.
These data, combined with northern analysis and SKCS data, indicate that additional copies of the puroindoline genes in hexaploid wheat increase the amount of Pina and Pinb expression and also increase friabilin and starch surface protein levels, with an end result being decreased kernel hardness.
RNA was extracted from the peach trees, purified to mRNA, and examined using Northern Analysis
Northern analysis was used to confirm that HDI increased message levels of these four genes and to further explore the dose dependence and kinetics of the response.
The following IMAGE (Integrated Molecular Analysis of Genomes and their Expression consortium) clones from the microarray were further studied by Northern analysis (Table 1).