More recently, quantitative reverse transcription PCR, Northern analysis
, and in situ hybridization have been adapted to quantify miRNAs.
The genes selected by differential screening were subjected to Northern analysis to confirm the differential expression of genes between the two genetic breeds of chickens.
Two genes were confirmed by Northern analysis to be differentially expressed in the livers of different lines of chickens.
RNA was extracted from the peach trees, purified to mRNA, and examined using Northern Analysis
was used to confirm that HDI increased message levels of these four genes and to further explore the dose dependence and kinetics of the response.