Nocardiopsis dassonvillei (strain 8r) belongs to the genus Nocardiopsis, a widespread group, which is versatile and pathogenic that produces a wide range of bioactive metabolites (28-29).
Strain 1r had a 99.7% similarity with Cellulomonas bogoriensis and strain 8r 99.69% with Nocardiopsis dassonvillei (Figure 1).
Two of those seven strains, one with 99.7% similarity with Cellulomonas bogoriensis and one with 99.69% similarity with Nocardiopsis dassonvillei showed cellulolytic activity as evidenced by the production of reducing sugars.
Complete genome sequence of Nocardiopsis dassonvillei type strain (IMRU 509(T)).
Cellulolytic activity (mU [ml.sup.-1]) and microbial growth (O.D.) of strain 8r Nocardiopsis dassonvillei at five different temperatures and pH 9.0 incubated for 60 days
Cellulolytic activity (mU [ml.sup.-1]) and microbial growth (O.D.) of strain 8r Nocardiopsis dassonvillei at different pH and 50[degrees]C incubated for 60 days
Culture of some of the bacteria predicted to have nitrilase gene (Shimwellia blattae, Runella slithyformis, Geodermatophilus obscurus, Nocardiopsis dassonvillei, Streptomyces albus, Flavobacterium indicum, Saccharomonospora viridis, Sphingopyxis alaskensis, and Gluconacetobacter diazotrophicus) was procured from Microbial Type Culture Collection (MTCC); Chandigarh Escherichia coli BL21 (DE3) from Invitrogen was used as negative control as this organism does not have nitrilase gene.
On the other hand, Geodermatophilus obscurus, Nocardiopsis dassonvillei, Runella slithyformis, and Streptomyces albus exhibited nitrilase activity for aromatic nitriles.