Rifamycin B is powerful, less toxic, and easily biodegradable antibacterial ansamycin antibiotic  and is produced by strain of Nocardia mediterranei. It bear distinct critical antibacterial activities , including tuberculosis, leprosy, and AIDS-related mycobacterial infections .
Nocardia mediterranei MTCC14 was obtained from the Microbial Type Culture Collection and Gene Bank (MTCC), Chandigarh, India, and was used in this study for rifamycin B production.
Plackett-Burman design test was applied to describe the significant variables responsible for production of rifamycin B from Nocardia mediterranei MTCC 14.
The complementary nutrients and physical parameters really advance concentration of rifamycin B of Nocardia mediterranei MTCC 14.
Hence, the effect of different nitrogen sources on rifamycin B production by Nocardia mediterranei MTCC 14 was studied at optimized SSF environment.
Analysis of variance for the rifamycin B produced from Nocardia mediterranei MTCC 14 obtained from this design was given in Table 6.
Optimum levels of production parameters for production of rifamycin B from Nocardia mediterranei MTCC14 was 9.87 g/kgds, which is very close to the predicted value of 10.35 g/kgds.
Response surface methodology was well used to optimize the production parameters for production of rifamycin B from Nocardia mediterranei MTCC 14.