Indeed, while MMP9 has been reported to be nitrosylated , it was colocalized with iNOS and S-nitrosylated proteins at the leading edge in trophoblast .
Similarly, actin, whose modifications of properties are requested for the rapid cadence of cytokinesis during early embryogenesis, is nitrosylated or sulfhydrated.
New techniques that are suitable for the diagnostic analysis of nitrosylated
proteins are now available and capable to reveal NO targets in plants.
Nitrosylated Hbs formed by this method were analyzed spectrophotometrically for the presence of heme-bound NO  and chemically for the presence of sulfhydryl-bound NO with the Saville reaction .
5, dashed line) but is rapidly converted to (ferrous) nitrosylated Hb (Fig.
To test whether the sulfhydryls of Hb H could be nitrosylated in an analogous fashion, we treated Hb H with SNAP, a nitrosothiol shown to be effective in transnitrosylation reactions .
Our data also suggest that Hb H is less susceptible to oxidation in the presence of nitrite (and presumably NO) since it has the ability to rapidly convert methemoglobin H to ferrous nitrosylated Hb H.
These products serve as "markers of oxidative stress," and they include carbonylated, nitrosylated
, and glycated proteins; aggregates due to crosslinking of protein molecules; products of lipid peroxidation (malondialdehyde, diene conjugates, hydroxynonenal, etc.
number D-23844, ThermoFisher Scientific, Waltham, MA, US), which becomes fluorescent when it is nitrosylated
by oxidation products of NO, was added to the H9C2 cells in a working concentration of 10 [micro]M and allowed to incubate at 37[degrees]C in dark for 30 min.