nitroblue tetrazolium

(redirected from Nitro blue tetrazolium chloride)
Also found in: Wikipedia.

ni·tro·blue tet·ra·zo·li·um (NBT),

a pale yellow dye that is converted on reduction to colored formazans in the histochemical demonstration of dehydrogenases; used in hematology for staining of neutrophils to help indicate the presence of bacterial infections.

nitroblue tetrazolium

a yellow dye converted to a blue color on reduction.

nitroblue tetrazolium test
used to measure the phagocytic activity of polymorphonuclear leukocytes by the amount of color change in the dye.
References in periodicals archive ?
The embryos were then blocked (1 hr) in 20% goat serum, incubated overnight in an antidigoxigenin antibody coupled to alkaline phosphatase (1:2,000) (Roche), washed extensively in PBS (at least 6 x 1 hr) and overnight in PBS, followed by a chromogenic nitro blue tetrazolium chloride (NBT) reaction [NBT/BCIP (5-bromo-4chloro-3-indolyl-phosphate, toluidine-salt) tablets, Roche] for 10-30 min, until color was visible.