The remaining ingredients (ascorbic acid, sorbic acid, nipagin
, formaldehyde, tetracycline hydrochloride, and vitamin solution) together with 100 mL remaining distilled water were mixed in a blender for approximately 2 min (18,000 rpm) when the bean mixture cooled to 60 [degrees]C.
The subjects were properly identified and conditioned inside flat bottomed glass tubes (27 ml) containing artificial diets based on corn flour (80 g), yeast (40 g), powdered glucose (100 g), agar (8 g), propionic acid (3 ml), Nipagin
(8 ml, in a 10% alcohol dilution) and 1 L of distilled water, plugged with hydrophillic cotton.
Tube Diet (Feeding) Tray Diet (Re-feeding) Ingredients Amount Ingredients Amount Sugar 50 g Sugar 270 g Soy bran 550 g Soy bran 400 g Wheat germ 50 g Wheat germ 100 g Nipagin
33 g Nipagin
25 g Ascorbic Acid 10 g Ascorbic Acid 5 g Choline Chloride 2 g Choline Chloride 2 g Formaldehyde 10 ml Formaldehyde 10 ml Vitamin solution 60 ml Vitamin solution 30 ml Caragenate 87 g Caragenate 105 g Ampicillin 750 mg Ampicillin 500 mg Vita gold[R] 3 ml Vita gold[R] 2 ml Flagyl[R] 2 pills Flagyl[R] 1 pill Yeast 205 g Acetic acid 40 ml Wesson's salts 22 g Water 4000 ml Water 4000 ml Table 2.
The vehicle (topical formulation) was comprised of an aqueous phase composed of 75.8% water and 4.0% propylene glycol and an organic phase composed of 17% Lanette cream, 3% hard paraffin, 0.15% Nipagin
, and 0.05% Nipasol, pH 6.86 [+ or -] 0.0215.
El contenido de la dieta fue 7.5% de polvo de olote, 7.5 de bagazo de cana, 4% de salvado de trigo, 12% azucar, 9% levadura, 1.7% acido citrico, 0.3% de benzoato de sodio, 0.4 de nipagin
, 57.6% de agua.
The newly hatched flies were maintained for about 3 days in an incubator with controlled temperature of 25[degrees]C and 30-50% humidity under a light/dark cycle of 12 h fed on standard medium (1% yeast w/v, 2% w/v sucrose, 1% w/v milk powder, 1% agar w/v, and 0.08% v/w nipagin
Newly hatched small tiny larvae visible to the naked eye were shifted to glass jars (10A-5 mm) having 30g standardized larval rearing diet: Wheat brain 100g, honey 25ml, L-Ascorbic acid [AnalaR 500g BDH laboratory supplies] 0.75g, methylparaben (Methyl 4-hydroxybenzoate, Nipagin
) 0.75g and yeast (Saf-instant(R) S.I.
vittella was reared following the method (PACopyrightrez-Guerrero et al., 2004) using semi-artificial diet comprising 350 ml water, 215 g dried kidney beans boiled in water, 32 g brewer's yeast, 12 g agar, 3 g ascorbic acid, 1.5 g benzoic acid, and 1.5 g nipagin
(PACopyrightrez-Guerrero et al., 2004).
John's Wort extract (extractum herba Hyperici), Triethanolamine, nipagin
, Carbopol 940 9 Reumabloc-Sun Boswelia Serrata Extract, Curcuma Wave Pharma Longa, Vitex negundo 10 Tone the Arnica tincture, sea buckthorn oil, tonic and camphor and spearmint oil relaxing cream 50 g Nr.
The company offers a broad portfolio of single actives and optimized blends under the well-known brand names Phenonip, Nipagin