Serum samples of 0.1 [micro]L stained with Ponceau S or nigrosin were adequate for quantitative densitometry or elution.
Ponceau S and nigrosin protein staining techniques were presented.
The letter, "Nigrosine the Forgotten Stain", was written in response to the claims of another author that nigrosin was as sensitive as gold stain for protein detection on CA.
He ended with a reference to an unpublished study in which he along with coworkers found that metallic gold stain for protein was at least 20 times more sensitive than nigrosin.
The eosin-nigrosin solution was prepared by dissolving 1.67 g eosin and 10 g nigrosin in 100 mL.
The eosin nigrosin exclusion and the hyposmotic swelling test were combined to a form a single test (HOS-EN test) to identify four types of spermatozoa according to membrane integrity and function.
A The addition of India ink or nigrosin
to CSF sediment and subsequent examination by light microscopy has been commonly used to detect encapsulated strains of Cryptococcus neoformans.
and Eosin stains was used to determine the percentage live count.
For a number of years, the India ink (nigrosin
) wet mount was commonly performed on CSF to detect the encapsulated yeast Cryptococcus.
The eosin nigrosin
staining technique was used to estimate live spermatozoa by following the standard protocol.
The staining solution for the one-step eosin-nigrosin staining contained 0.67 g eosin Y, 0.9 g sodium chloride and 10 g of nigrosin
. Aliquots of each 6 samples (50 [micro]l) mixed with same amount of solution in micro tube.