On the other hand, nuclear chromatin staining by DAPI showed no change of chromatins in clivorine-treated PCI 2 cells (5 to 25 [micro]M); while neurofilament
, staining by NF68, showed significantly decrease of neurite length in PC12 cells exposed to 25 [micro]M divorine (Fig.
Alpha-internexin is structurally and functionally associated with the neurofilament
triplet protein the mature CNS.
The core of the new discovery is the source of these tangles: a shortage of one of the three proteins in the neurofilament
In response to serum starvation or treatment with retinoic acid (RA) or dibutyryl cyclic AMP, N2a cells undergo neuronal differentiation characterized by cell cycle arrest, neurite outgrowth, and up-regulation of neurofilament
(NF) proteins (Dasgupta and Milbrandt 2007).
It is now well established that the axonal calibre, hence the nerve conduction velocity, is governed by the phosphorylation status of lycine-serine-proline (KSP) repeats of--COOH termini of high [NF-H; 200 kilo dalton (kDa)] and medium (NF-M; 150 kDa) molecular weight subunits of neurofilament
Methods: Different neuroanatomical areas including frontal cortex (FC), cerebellum (CB), caudate nucleus (CD) and substantia nigra (SN) from autopsy human brains (n=9) with varying PMI (4-18 h) were analyzed for pH, protein insolubility, protein oxidation/nitration and protein expression of glial fibrillary acidic protein (GFAP), synatophysin and neurofilament
Similar protocols were performed with antibodies against Actin smooth muscle specific and neurofilaments
(NeoMakers ref RB 9010-R7) and the androgen receptor (LabVision CO.
This truncated form of the protein is thought to induce neuronal inclusions that are similar to neurofibrillatory tangles, containing phosphorylated tau and high-molecular-weight neurofilaments
Within the cytoplasm are neurofilaments
, which provide structure, and microtubules, which supply the mechanism for rapid vesicle transport (Schnapp, Vale, Sheetz, & Reese, 1987).
2002) demonstrated that NEFL mutant neurofilaments
assembly and their axonal transport in mammalian cells and neurons is disrupted.
The electronmicrographs of EMF exposed group showed axonal remyelination, condensed state of mitochondria, and evenly distributed neurofilaments
and microtubules, whereas the EMF unexposed group showed axonal demyelination, mitochondria in an orthodox state of conf ormation and an uneven distribution of neurofilaments
Lewy bodies are rounded, cosinophilic intracytoplasmic neuronal inclusions composed of altered neurofilaments