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For example, neurofilament staining will highlight entrapped axons in the middle of an infiltrative tumor.
The sections from lowest PMI (ID B-183) and longest PMI (ID T-144) were immunostained with antibodies to GFAP (Glial marker, 1:200) and neurofilament (axonal marker, 1:100) to note if there is any difference in staining character.
But in their mice at least, says study leader Jean-Pierre Julien of McGill University in Montreal, the neurofilaments indeed play the villain.
Additionally, 5-[micro]m sections were taken from the paraffin blocks and stained using antibodies against Melan-A (prediluted, monoclonal, catalog number 790-2990; Ventana Medical Systems, Tucson, Arizona), S100 protein (prediluted, polyclonal, catalog number 760-2523; Ventana Medical), and neurofilament (prediluted, clone 2F11, monoclonal, catalog number 760-2661; Ventana Medical) following the manufacturer's protocols in a BenchMark XT automated slide stainer (Ventana Medical).
progressive changes in phosphorylation states of paired helical filaments and neurofilaments.
The surgical specimen was tested for S- 100 protein, neurofilaments, neuron-specific enolase, CD56, CD57, and synaptophysin (table).
FSH - (1->3)-L-fucosyltransferase - Gastrin-Releasing Peptide (GRP) - GDCFP-15 - Glucagon - Glycoamines - H23 - Her-2 - Human Carcinoma Antigen - HPA - HSP27 - Intermediate Filaments - Cytokeratins/CK18/Cyfra 21-1 - Desmin - Gliofibrillary Acid Protein - Neurofilaments - Vimentin - KA 93 - Kinases - KP16D3 - LAI - Leukocyte Common Antigen - Lewis Antigens - Lysophosphatidic Acid (LPA) - Ma 695/Ma 552 - MABDF3 - MAG - ME1 - Minactivin - MN/CA9 - MSA - Mucin Cancer Antigen (MCA) - Multiple Tumor Suppressor 1 - Myosin - NEA-130 - NMP22 - OA-519 - Opiod Peptides - P-glycoprotein - Pancreatic Oncofetal Antigen (POA) - Placental Lactogen - PR92 - Proliferative Index, Ki-67
11) Merkel cell carcinoma expresses a wide range of neuroendocrine markers such as neurofilaments, neuron-specific enolase, chromogranin, synaptophysin (Figure 3), bombesin, somatostatin, vasoactive intestinal peptide, and proconvertases PC1/ PC3 and PC2.
Immunohistochemical tests were positive for neuron-specific enolase, immunoreactive neurofilaments, CD57 (Leu-7), and synaptophysin.
Her research and publications include: Unraveling the mechanisms involved in motor neuron degeneration in ALS, Annual Review Neuroscience; Amyotrophic lateral sclerosis: from disease mechanisms to therapies, Biotechniques; Toxicity of ALS-linked SOD1 mutants, Science; Aggregation and motor neuron toxicity of an ALS-linked SOD1 mutant independent from wild-type SOD1, Science; and Absence of neurofilaments reduces the selective vulnerability of motor neurons and slows disease caused by a familial amyotrophic lateral sclerosis-linked superoxide dismutase 1 mutant, Proceeding of the National Academy of Sciences.
16) In addition, neurofilaments, neurotubules and, occasionally, Schwann-like cells can be seen.
University of California, San Diego Neurofilaments, SOD1 and Lou Gehrig's Disease -- Elaine Fuchs, Ph.