Mapping of the human NMDAR2B
receptor subunit gene (GRIN2B) to chromosome 12p12.
In ZnT3 knockout mice, the addition of metal chaperones results in restoration of expression of the synaptic proteins PSD-95, AMPAR, and NMDAR2b
, due to the restitution of hippocampal zinc content .
Coexpression of MLCK and NMDA receptor was also significantly higher than that in group A, which showed a statistically significant difference (P < 0.05) (Figure 2, Supplementary Table 1 in Supplementary Material available online at http://dx.doi.org/10.1155/2015/484721, the expression of MLCK showed in red fluorescence, the expression of NMDAR2B
in green fluorescence, and the coexpression in yellow fluorescence.).
Following the transfer onto PVDF membranes, the membranes were blocked by Tris--buffered saline containing 7.5% nonfat dry milk, 10 mmol/L Tris--HCI (pH 7.5), and 0.15% Tween-20 for 2 h, then incubated over night at 4 [degrees]C with primary antibodies: NMDAR2B
, 1:100 from Abcam, p44/42 MAPK (Erkl /2) (137F5) 1:500; phosphorylation of ERK (pERK)(Thr202/yr204), 1:500; CREB (48H2) 1:500; Phospho-CREB (Ser133) (87G3) 1:500, from Cell Signaling Technology (Danvers, MA, USA); the membranes were washed with PBS-Tween and then incubated with a peroxidase-conjugated secondary antibody (1:2000; ZSGB-B10) at 37 [degrees]C for 3h.
However, Pascual and colleagues (2009) demonstrated that in adolescent rats chronically treated with ethanol, two neurotransmitter receptors--dopamine receptor 2 (DRD2) and glutamate receptor (NMDAR2B
)--show lower levels of a chemical modification (i.e., phosphorylation) in the prefrontal cortex compared with adults chronically treated with ethanol.
Different trends in modulation of NMDAR1 and NMDAR2B
gene expression in cultured cortical and hippocampal neurons after lead exposure.