GRIN2B

(redirected from NMDAR2B)

GRIN2B

A gene on chromosome 12p12 that encodes an epsilon subunit of N-methyl-D-aspartate (NMDA) receptors, which belong to the glutamate receptor channel superfamily. NMDA receptors consist of multiple subunits arranged to form a ligand-gated ion channel, which play a key role in long-term potentiation and the plasticity of synapses (central to memory and learning). GRIN2B forms a complex with GRIN1, GRIN3A and PPP2CB; it interacts with HIP1, NETO1, MAGI3 and DAPK1, and with the PDZ domains of INADL and DLG4. It is highly expressed in the fronto-parieto-temporal cortex and hippocampus pyramidal cells.

Molecular pathology
Defects in GRIN2B cause mental retardation autosomal dominant type 8.
References in periodicals archive ?
In ZnT3 knockout mice, the addition of metal chaperones results in restoration of expression of the synaptic proteins PSD-95, AMPAR, and NMDAR2b, due to the restitution of hippocampal zinc content [113].
Coexpression of MLCK and NMDA receptor was also significantly higher than that in group A, which showed a statistically significant difference (P < 0.05) (Figure 2, Supplementary Table 1 in Supplementary Material available online at http://dx.doi.org/10.1155/2015/484721, the expression of MLCK showed in red fluorescence, the expression of NMDAR2B in green fluorescence, and the coexpression in yellow fluorescence.).
Following the transfer onto PVDF membranes, the membranes were blocked by Tris--buffered saline containing 7.5% nonfat dry milk, 10 mmol/L Tris--HCI (pH 7.5), and 0.15% Tween-20 for 2 h, then incubated over night at 4 [degrees]C with primary antibodies: NMDAR2B, 1:100 from Abcam, p44/42 MAPK (Erkl /2) (137F5) 1:500; phosphorylation of ERK (pERK)(Thr202/yr204), 1:500; CREB (48H2) 1:500; Phospho-CREB (Ser133) (87G3) 1:500, from Cell Signaling Technology (Danvers, MA, USA); the membranes were washed with PBS-Tween and then incubated with a peroxidase-conjugated secondary antibody (1:2000; ZSGB-B10) at 37 [degrees]C for 3h.
However, Pascual and colleagues (2009) demonstrated that in adolescent rats chronically treated with ethanol, two neurotransmitter receptors--dopamine receptor 2 (DRD2) and glutamate receptor (NMDAR2B)--show lower levels of a chemical modification (i.e., phosphorylation) in the prefrontal cortex compared with adults chronically treated with ethanol.
Different trends in modulation of NMDAR1 and NMDAR2B gene expression in cultured cortical and hippocampal neurons after lead exposure.