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The enzyme was obtained with specific activity of 1291 nkat.mg-1 of protein and recovery of 0.7%.
A 222 - folds purification was resulted with specific activity of 1291 nkat.mg-1 of protein.
The specific activity was expressed as nkat.mg-1 of protein.
To examine o-diphenol dependence of proteolytic inhibition, we combined extracts of Control- and PPO-Alfalfa to contain a fixed level (4.17 nkat [mL.sup.-1]) of PPO activity at a total protein concentration of 2 mg [mL.sup.-1].
PPO- and Control-Alfalfa extract were combined to contain 4.17 nkat [mL.sup.-1] PPO activity at a final protein concentration of 2 mg [mL.sup.-1].