PCSK1

(redirected from NEC1)

PCSK1

A gene on chromosome 5q15-q21 that encodes a peptidase S8 family convertase involved in processing pairs of basic amino acid residues located on prohormones and other protein precursors (e.g., POMC, renin, enkephalin, dynorphin, somatostatin and insulin).

Molecular pathology
Defects in PCSK1 cause proprotein convertase 1 deficiency.
References in periodicals archive ?
The cells were treated with Z-IETD-FMK (40 [micro]M), Nec1 (50 [micro]M), or Z-IETD-FMK (40 [micro]M) and Nec-1 (50 [micro]M) for 1 h, after which they were treated with TNF-[alpha] (20 ng/mL) for an additional 12 or 24 h, and protein lysates were extracted in radioimmunoprecipitation (RIPA) lysis buffer containing 4% protease inhibitor, 4% phosphorylase inhibitor, and 1% PMSF.
Sorafenib (SF) necrostatin 1 (Nec1), and 1-methyl-D-tryptophan (1-D-M-T) were purchased from VWR (Radnor, PA).
Necrostatin 1 (Nec1) Partially Prevented the Effects of VP + SF on Cell Viability.
However, pharmacological evidence indicates that necroptosis and, to a lesser extent, apoptosis play a major role in cell death induced by VP + SF since (1) the inhibitory effect of VP + SF on cell viability could be partially prevented by Nec1 in both cells growing under PPSS and tumorspheres.
Abbreviations RCCs: Routine culture conditions PPSS: Prolonged period of serum starvation FTs: Floating tumorspheres VP: Verapamil SF: Sorafenib CQ: Chloroquine zVAD: z-VAD-FMK Nec1: Necrostatin 1 1-M-D-T: 1-Methyl-D-tryptophan.
Therefore, we investigated whether Nec1 affects hepatic RIP1 expression, using western blotting and immunocytochemical staining in mice with ConA-induced liver injury.
As shown in Figure 4(a), the increase in intrahepatic levels of TNF-[alpha] in response to ConA was prevented by pretreatment with Nec1. Therefore, the prevention of ConA-induced liver injury by Nec-1 is associated with the inhibition of the release of proinflammatory cytokines, such as TNF-[alpha].
The Centauro 2 will be fully integrated in the Italian Army digitised battlefield network known as 'Forza NEC1 and will thus be equipped with the Siccona command, control and navigation system.